Single microcystin exposure impairs the hypothalamic-pituitary-gonadal axis at different levels in female rats.

Autor: Dos Santos FCF; Department of Morphology, Federal University of Espírito Santo, 290440-090, Vitória, Brazil., Lima GFC; Department of Morphology, Federal University of Espírito Santo, 290440-090, Vitória, Brazil., Merlo E; Department of Morphology, Federal University of Espírito Santo, 290440-090, Vitória, Brazil., Januario CF; Department of Morphology, Federal University of Espírito Santo, 290440-090, Vitória, Brazil., Miranda-Alves L; Experimental Endocrinology Research, Development and Innovation Group, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, 21941-904, Ilha do Governador, Brazil., Miranda RA; Laboratory of Endocrine Physiology, Biology Institute, State University of Rio de Janeiro, RJ, Brazil., Lisboa PC; Laboratory of Endocrine Physiology, Biology Institute, State University of Rio de Janeiro, RJ, Brazil., Graceli JB; Department of Morphology, Federal University of Espírito Santo, 290440-090, Vitória, Brazil. Electronic address: jbgraceli@gmail.com.
Jazyk: angličtina
Zdroj: Molecular and cellular endocrinology [Mol Cell Endocrinol] 2024 May 15; Vol. 586, pp. 112203. Date of Electronic Publication: 2024 Mar 13.
DOI: 10.1016/j.mce.2024.112203
Abstrakt: Microcystin (MC) is most common cyanobacterial toxin. Few studies have evaluated the MC effects on the hypothalamic-pituitary-gonadal (HPG) axis and metabolic function. In this study, we assessed whether MC exposure results in HPG axis and metabolic changes. Female rats were exposed to a single dose of MC at environmentally relevant levels (5, 20 and 40 μg/kg). After 24 h, we evaluated reproductive and metabolic parameters for 15 days. MC reduced the hypothalamic GnRH protein expression, increased the pituitary protein expression of GnRHr and IL-6. MC reduced LH levels and increased FSH levels. MC reduced the primary follicles, increased the corpora lutea, elevated levels of anti-Müllerian hormone (AMH) and progesterone, and decreased estrogen levels. MC increased ovarian VEGFr, LHr, AMH, ED1, IL-6 and Gp91-phox protein expression. MC increased uterine area and reduced endometrial gland number. A blunted estrogen-negative feedback was observed in MC rats after ovariectomy, with no changes in LH levels compared to intact MC rats. Therefore, these data suggest that a MC leads to abnormal HPG axis function in female rats.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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