Characterisation of protective vaccine antigens from the thiol-containing components of excretory/secretory material of Ostertagia ostertagi.

Autor: Price DRG; Moredun Research Institute, Pentlands Science Park, Midlothian EH26 0PZ, UK. Electronic address: dan.price@moredun.ac.uk., Steele P; Moredun Research Institute, Pentlands Science Park, Midlothian EH26 0PZ, UK., Frew D; Moredun Research Institute, Pentlands Science Park, Midlothian EH26 0PZ, UK., McLean K; Moredun Research Institute, Pentlands Science Park, Midlothian EH26 0PZ, UK., Androscuk D; Moredun Research Institute, Pentlands Science Park, Midlothian EH26 0PZ, UK., Geldhof P; Laboratory of Parasitology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Sciences, Ghent University, Belgium., Borloo J; Laboratory of Parasitology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Sciences, Ghent University, Belgium., Albaladejo JP; Biomathematics and Statistics Scotland, JCMB, The King's Buildings, Peter Guthrie Tait Road, Edinburgh, Scotland, UK; Department of Computer Science, Applied Mathematics and Statistics, University of Girona, Girona, Spain., Nisbet AJ; Moredun Research Institute, Pentlands Science Park, Midlothian EH26 0PZ, UK., McNeilly TN; Moredun Research Institute, Pentlands Science Park, Midlothian EH26 0PZ, UK.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2024 Jun; Vol. 328, pp. 110154. Date of Electronic Publication: 2024 Mar 01.
DOI: 10.1016/j.vetpar.2024.110154
Abstrakt: Previous vaccination trials have demonstrated that thiol proteins affinity purified from Ostertagia ostertagi excretory-secretory products (O. ostertagi ES-thiol) are protective against homologous challenge. Here we have shown that protection induced by this vaccine was consistent across four independent vaccine-challenge experiments. Protection is associated with reduced cumulative faecal egg counts across the duration of the trials, relative to control animals. To better understand the diversity of antigens in O. ostertagi ES-thiol we used high-resolution shotgun proteomics to identify 490 unique proteins in the vaccine preparation. The most numerous ES-thiol proteins, with 91 proteins identified, belong to the sperm-coating protein/Tpx/antigen 5/pathogenesis-related protein 1 (SCP/TAPS) family. This family includes previously identified O. ostertagi vaccine antigens O. ostertagi ASP-1 and ASP-2. The ES-thiol fraction also has numerous proteinases, representing three distinct classes, including: metallo-; aspartyl- and cysteine proteinases. In terms of number of family members, the M12 astacin-like metalloproteinases, with 33 proteins, are the most abundant proteinase family in O. ostertagi ES-thiol. The O. ostertagi ES-thiol proteome provides a comprehensive database of proteins present in this vaccine preparation and will guide future vaccine antigen discovery projects.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Crown Copyright © 2024. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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