Protocol to visualize and quantify the COPII concentration and anterograde transport of nascent G protein-coupled receptors.
| Autor: | Xu X; Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA. Electronic address: xinxu@augusta.edu., Wu G; Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA. Electronic address: guwu@augusta.edu. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2024 Jun 21; Vol. 5 (2), pp. 102955. Date of Electronic Publication: 2024 Mar 13. |
| DOI: | 10.1016/j.xpro.2024.102955 |
| Abstrakt: | Here, we present a protocol for visualization and quantification of the recruitment of newly synthesized G protein-coupled receptors (GPCRs) to coat protein complex II vesicles and GPCR transport from the endoplasmic reticulum through the Golgi to the cell surface in the retention using the selective hooks assay. We describe steps for plasmid construction, cell transfection, transport synchronization, confocal microscope imaging, and quantification. This protocol is also applicable for studying the transport of non-GPCR cargoes. For complete details on the use and execution of this protocol, please refer to Xu et al. 1 , 2 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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