Con-FLIC: concurrent measurement of feeding behaviors and food consumption in Drosophila at single-fly resolution.

Autor:	Opoola M; Department of Biology, University of Louisville, Louisville, Kentucky, United States., Fitzgerald L; Department of Biology, University of Louisville, Louisville, Kentucky, United States., Hwangbo DS; Department of Biology, University of Louisville, Louisville, Kentucky, United States.
Jazyk:	angličtina
Zdroj:	MicroPublication biology [MicroPubl Biol] 2024 Feb 28; Vol. 2024. Date of Electronic Publication: 2024 Feb 28 (Print Publication: 2024).
DOI:	10.17912/micropub.biology.001128
Abstrakt:	Accurate quantification of food intake and feeding behaviors are essential for understanding various biological processes, such as metabolism and aging. Currently, no methods allow for the concurrent measurement of both parameters for the same individual flies. Here, we couple Con-Ex ( Con sumption- Ex cretion) and FLIC ( F ly L iquid-Food I nteraction C ounter), previously developed to measure food consumption and various feeding behaviors, respectively, into a single platform that we named Con-FLIC. Using starvation as a known condition that changes food intake and feeding behaviors, we validate that Con-FLIC enables concurrent measurement of feeding behaviors and food intake in Drosophila at a single-fly resolution. We expect that Con-FLIC will be an easy non-invasive option to quantify food consumption and feeding behaviors concurrently in the same individual flies. Competing Interests: The authors declare that there are no conflicts of interest present. (Copyright: © 2024 by the authors.)
Databáze:	MEDLINE
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