An optrode array for spatiotemporally-precise large-scale optogenetic stimulation of deep cortical layers in non-human primates.

Autor: Clark AM; Department of Ophthalmology and Visual Science, Moran Eye Institute, University of Utah, Salt Lake City, UT, USA., Ingold A; Department of Ophthalmology and Visual Science, Moran Eye Institute, University of Utah, Salt Lake City, UT, USA., Reiche CF; Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, UT, USA., Cundy D 3rd; Department of Ophthalmology and Visual Science, Moran Eye Institute, University of Utah, Salt Lake City, UT, USA., Balsor JL; Department of Ophthalmology and Visual Science, Moran Eye Institute, University of Utah, Salt Lake City, UT, USA., Federer F; Department of Ophthalmology and Visual Science, Moran Eye Institute, University of Utah, Salt Lake City, UT, USA., McAlinden N; SUPA, Institute of Photonics, Department of Physics, University of Strathclyde, Glasgow, UK., Cheng Y; SUPA, Institute of Photonics, Department of Physics, University of Strathclyde, Glasgow, UK., Rolston JD; Departments of Neurosurgery and Biomedical Engineering, University of Utah, Salt Lake City, UT, USA.; Department of Neurosurgery, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA., Rieth L; Mechanical and Aerospace Engineering, West Virginia University, Morgantown, WV, USA.; Feinstein Institute for Medical Research, Manhasset, NY, USA., Dawson MD; SUPA, Institute of Photonics, Department of Physics, University of Strathclyde, Glasgow, UK., Mathieson K; SUPA, Institute of Photonics, Department of Physics, University of Strathclyde, Glasgow, UK., Blair S; Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, UT, USA. blair@ece.utah.edu., Angelucci A; Department of Ophthalmology and Visual Science, Moran Eye Institute, University of Utah, Salt Lake City, UT, USA. alessandra.angelucci@hsc.utah.edu.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2024 Mar 14; Vol. 7 (1), pp. 329. Date of Electronic Publication: 2024 Mar 14.
DOI: 10.1038/s42003-024-05984-2
Abstrakt: Optogenetics has transformed studies of neural circuit function, but remains challenging to apply to non-human primates (NHPs). A major challenge is delivering intense, spatiotemporally-precise, patterned photostimulation across large volumes in deep tissue. Such stimulation is critical, for example, to modulate selectively deep-layer corticocortical feedback circuits. To address this need, we have developed the Utah Optrode Array (UOA), a 10×10 glass needle waveguide array fabricated atop a novel opaque optical interposer, and bonded to an electrically addressable µLED array. In vivo experiments with the UOA demonstrated large-scale, spatiotemporally precise, activation of deep circuits in NHP cortex. Specifically, the UOA permitted both focal (confined to single layers/columns), and widespread (multiple layers/columns) optogenetic activation of deep layer neurons, as assessed with multi-channel laminar electrode arrays, simply by varying the number of activated µLEDs and/or the irradiance. Thus, the UOA represents a powerful optoelectronic device for targeted manipulation of deep-layer circuits in NHP models.
(© 2024. The Author(s).)
Databáze: MEDLINE
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