Chromene-chromene Schiff base as a fluorescent chemosensor for Th 4+ and its application in bioimaging of Caenorhabditis elegans.

Autor: Dua A; Department of Chemistry, M.M Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala 133 207, Haryana, India., Saini P; Department of Chemistry, M.M Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala 133 207, Haryana, India., Goyal S; Department of Chemistry, M.M Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala 133 207, Haryana, India., Selvam P; Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore 632 014, Tamil Nadu, India., Ashok Kumar SK; Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore 632 014, Tamil Nadu, India., Thiruppathi G; Unit of Nematology, Department of Zoology, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India., Sundararaj P; Unit of Nematology, Department of Zoology, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India., Sharma HK; Department of Chemistry, M.M Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala 133 207, Haryana, India., Kumar Ramasamy S; Department of Chemistry, M.M Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala 133 207, Haryana, India. Electronic address: selvachemst@gmail.com.
Jazyk: angličtina
Zdroj: Methods (San Diego, Calif.) [Methods] 2024 May; Vol. 225, pp. 28-37. Date of Electronic Publication: 2024 Mar 12.
DOI: 10.1016/j.ymeth.2024.03.002
Abstrakt: The manuscript presents the synthesis of a new di-chromene Schiff base (COM-CH) by combining 7-(diethylamino)-2-oxo-2H-chromene-3-carbohydrazide and 4-oxo-4H-chromene-3-carbaldehyde, and its characterization using various analytical techniques. The probe COM-CH functional group contains a hard donor atom that selectively complexes with Th 4+ ions. This report investigated COM-CH's sensing ability towards Th 4+ chromogenic and fluorogenic methods in ACN: H 2 O (8:2, v/v) with Th 4+ ions. The COM-CH-Th 4+ complex was excited at 430 nm, resulting in a bright emission band at 475 nm with a 45 nm Stokes shift. The COM-CH probe demonstrated the highest performance at pH 4.0 to 8.0, with a sensitivity of 18.7 nM. The complex formation of COM-CH with Th 4+ was investigated using NMR, FTIR spectrometry, and density functional theory calculations. The COM-CH and Th 4+ are bound with 2:1 stoichiometry and an association constant of 1.92 × 10 8 M -2 . The probe's performance enabled the analysis of monazite sand and water samples for Th 4+ content. The probe successfully detected Th 4+ content in Caenorhabditis elegans, marking the first Th 4+ detection in animal models.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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