Hepatic Effects of Low-Carbohydrate Diet Associated with Different Lipid Sources: Insights into Oxidative Stress, Cytotoxicity, and Epigenetic Markers in a mouse Model of Obesity.

Autor:	Santamarina AB; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo (UNIFESP), Santos, São Paulo, Brazil., Sertorio MN; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo (UNIFESP), Santos, São Paulo, Brazil., Mennitti LV; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo (UNIFESP), Santos, São Paulo, Brazil., Souza EA; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo (UNIFESP), Santos, São Paulo, Brazil., Souza DV; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo (UNIFESP), Santos, São Paulo, Brazil., Ribeiro DA; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo (UNIFESP), Santos, São Paulo, Brazil., Pisani LP; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo (UNIFESP), Santos, São Paulo, Brazil. Electronic address: pisani@unifesp.br.
Jazyk:	angličtina
Zdroj:	The Journal of nutrition [J Nutr] 2024 May; Vol. 154 (5), pp. 1517-1531. Date of Electronic Publication: 2024 Mar 12.
DOI:	10.1016/j.tjnut.2024.03.007
Abstrakt:	Background: Low-carbohydrate and high-fat diet (LCHF) models have been widely explored as alternatives for treating obesity and promoting weight loss. Their effect is attributed to the change in energy substrate that stimulates ketogenic pathways that can metabolically overload the liver. However, little has been studied about the impact of lipid sources prioritized in the LCHF diet. Objectives: This study aims to evaluate the impact of different fat sources in the LCHF diet on markers of liver injury, oxidative stress, and epigenetics in obesity. Methods: Adult male mice were initially induced to obesity by a high-fat and high-sugar diet for 10 wk. Subsequently, they underwent a weight-loss treatment intervention involving an LCHF diet with various sources of fats, including saturated, omega-3 (ω-3) (n-3), omega-6 (ω-6) (n-6), and omega-9 (ω-9) (n-9). At the end of the treatment, markers of liver injury, oxidative stress, and epigenetics were evaluated. Results: The LCHF diet was effective in inducing weight loss. However, unsaturated lipid sources (omegas) exhibited superior outcomes. Specifically, the ω-9 group displayed diminished oxidative stress concentrations and decreased markers of liver injury. The ω-3 group demonstrated efficacy in modulating epigenetic markers, thereby reducing oxidative stress, mutagenicity, and markers of liver injury. Correlation tests demonstrated that there was an interaction between the activity of antioxidants and epigenetic enzymes. Conclusions: Our results suggest that LCHF diets associated with ω-3 and ω-9 have the potential for weight loss and liver health recovery in obesity through antioxidant and epigenetic mechanisms. (Copyright © 2024 American Society for Nutrition. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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