Apilarnil ameliorates Bisphenol A-induced testicular toxicity in adult male rats via improving antioxidant potency and PCNA expression.

Autor: Elashal MH; Department of Chemistry, Faculty of Science, Menoufia University, Shebin El-Kom 31100107, Egypt., Abd El-Wahed AA; Department of Bee Research, Plant Protection Research Institute, Agricultural Research Centre, Giza 12627, Egypt. Electronic address: aidaabd.elwahed@arc.sci.eg., Mohamed MA; Pathology Department, Faculty of Veterinary Medicine, Menoufia University, Shebin El-Kom 31100107, Egypt. Electronic address: mostafaabdelgaber@vet.menofia.edu.eg., Hamad R; Pathology Department, Faculty of Veterinary Medicine, Menoufia University, Shebin El-Kom 31100107, Egypt., Abd Eldaim MA; Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Menoufia University, Shebin El-Kom 31100107, Egypt. Electronic address: mabroukattia@vet.menofia.edu.eg., Khalifa SAM; Psychiatry and Neurology Department, Capio Saint Göran's Hospital, Sankt Göransplan 1, Stockholm 112 19, Sweden; International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang 212013, China. Electronic address: shaden.khalifa@regionstockholm.se., Aldahmash B; Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia. Electronic address: Baldhmash@ksu.edu.sa., El-Seedi HR; Department of Chemistry, Faculty of Science, Islamic University of Madinah, Madinah 42351, Saudi Arabia. Electronic address: hesham@kth.se., El-Aarag B; Biochemistry Division, Chemistry Department, Faculty of Science, Menoufia University, Shebin El-Koom 31100107, Egypt.
Jazyk: angličtina
Zdroj: Reproductive toxicology (Elmsford, N.Y.) [Reprod Toxicol] 2024 Apr; Vol. 125, pp. 108570. Date of Electronic Publication: 2024 Mar 12.
DOI: 10.1016/j.reprotox.2024.108570
Abstrakt: Apilarnil, a bee-derived product originating from drone larvae, offers a range of advantageous properties for both humans and animals. It functions as an antioxidant, provides neuroprotection, boosts fertility, and has antiviral capabilities. Additionally, it is a provider of androgenic hormones. These beneficial functions are supported by its chemical composition, which comprises mineral salts, vitamins, carbs, lipids, hormones, and amino acids. The current study aimed to evaluate the ameliorative effect of apilarnil against Bisphenol A (BPA)-induced testicular toxicity in male adult rats. Forty-eight Wistar albino rats were randomly classified into six groups. The first, second, and third received olive oil, BPA at a dose of 50 mg/kg body weight (bwt), and apilarnil at a dose of 0.6 g/kg bwt, respectively. The fourth, fifth, and sixth groups received apilarnil with, before, or after BPA administration, respectively. Phytochemical analysis using included linear ion trap-ultra-performance liquid chromatography-tandem mass spectrometry (LTQ-UPLC-MS/MS) and global natural products social molecular networking (GNPS) revealed the presence of lysine, 10-hydroxy-(E)-2-dodecenoic acid, apigenin7-glucoside, testosterone, progesterone, and campesterol. BPA administration decreased serum level of follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone, glutathione (GSH) concentration, total sperm count, motility, and vitality. Additionally, BPA increased sperm abnormalities, malondialdehyde concentration (MDA), and decreased proliferating cell nuclear antigen (PCNA) expression. The treatment with apilarnil ameliorated BPA reproductive toxicity in rats which was indicated by increased serum testosterone levels, normalized serum levels of FSH and LH, and concentration of MDA and GSH activity. Moreover, apilarnil improved sperm count, motility, morphology, and PCNA expression. Apilarnil was found to enhance reproductive hormones, MDA levels, antioxidant activity, and PCNA expression.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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