Macrophage immunophenotypes in Jorge Lobo's disease and lepromatous leprosy- A comparative study.
Autor: | Sasso BM; State University of Campinas (UNICAMP), Campinas, Brazil. Electronic address: anatpat@unicamp.br., Vallarelli A; State University of Campinas (UNICAMP), Campinas, Brazil., Rosa PS; Lauro de Souza Lima Institute (ILSL), Bauru, Brazil., Belone A; Lauro de Souza Lima Institute (ILSL), Bauru, Brazil., Velho P; State University of Campinas (UNICAMP), Campinas, Brazil., Cintra ML; State University of Campinas (UNICAMP), Campinas, Brazil. |
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Jazyk: | angličtina |
Zdroj: | Microbial pathogenesis [Microb Pathog] 2024 May; Vol. 190, pp. 106610. Date of Electronic Publication: 2024 Mar 13. |
DOI: | 10.1016/j.micpath.2024.106610 |
Abstrakt: | Jorge Lobo's disease (JLD) and lepromatous leprosy (LL) share several clinical, histological and immunological features, especially a deficiency in the cellular immune response. Macrophages participate in innate and adaptive inflammatory immune responses, as well as in tissue regeneration and repair. Macrophage function deficiency results in maintenance of diseases. M1 macrophages produce pro-inflammatory mediators and M2 produce anti-inflammatory cytokines. To better understand JLD and LL pathogenesis, we studied the immunophenotype profile of macrophage subtypes in 52 JLD skin lesions, in comparison with 16 LL samples, using a panmacrophage (CD68) antibody and selective immunohistochemical markers for M1 (iNOS) and M2 (CD163, CD204) responses, HAM56 (resident/fixed macrophage) and MAC 387 (recently infiltrating macrophage) antibodies. We found no differences between the groups regarding the density of the CD163, CD204, MAC387+ immunostained cells, including iNOS, considered a M1 marker. But HAM56+ cell density was higher in LL samples. By comparing the M2 and M1 immunomarkers in each disease separately, some other differences were found. Our results reinforce a higher M2 response in JLD and LL patients, depicting predominant production of anti-inflammatory cytokines, but also some distinction in degree of macrophage activation. Significant amounts of iNOS + macrophages take part in the immune milieu of both LL and JLD samples, displaying impaired microbicidal activity, like alternatively activated M2 cells. Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Maria Leticia Cintra reports financial support was provided by State of Sao Paulo Research Foundation. Maria Leticia Cintra reports financial support was provided by Coordination of Higher Education Personnel Improvement. Andrelou Fralete Ayres Vallarelli reports was provided by Brazilian Society of Dermatology São Paulo State Regional. Paulo Eduardo Neves Ferreira Velho reports financial support was provided by National Council for Scientific and Technological Development. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
Databáze: | MEDLINE |
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