Expression of IZUMO1 and JUNO in the gonads of domestic cats (Felis catus).

Autor: Sanguansook P; Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand., Rodprasert W; Veterinary Pharmacology and Stem Cell Research Laboratory, Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Veterinary Stem Cell and Bioengineering Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand., Sawangmake C; Veterinary Pharmacology and Stem Cell Research Laboratory, Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Veterinary Stem Cell and Bioengineering Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Department of Veterinary Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand., Gimeno L; Department of Human Anatomy and Psychobiology, IMIB-Pascual Partilla Institute, University of Murcia, 30120, Murcia, Spain., Ferran JL; Department of Human Anatomy and Psychobiology, IMIB-Pascual Partilla Institute, University of Murcia, 30120, Murcia, Spain., Sòria-Monzó P; Department of Cell Biology and Histology, Faculty of Medicine, Campus de Ciencias de La Salud, University of Murcia, Campus Mare Nostrum (CMN), 30120, Murcia, Spain; Instituto Murciano de Investigación Biosanitaria Pascual Parrilla (IMIB), Campus de Ciencias de La Salud, 30120, Murcia, Spain., Avilés M; Department of Cell Biology and Histology, Faculty of Medicine, Campus de Ciencias de La Salud, University of Murcia, Campus Mare Nostrum (CMN), 30120, Murcia, Spain; Instituto Murciano de Investigación Biosanitaria Pascual Parrilla (IMIB), Campus de Ciencias de La Salud, 30120, Murcia, Spain., Izquierdo Rico MJ; Department of Cell Biology and Histology, Faculty of Medicine, Campus de Ciencias de La Salud, University of Murcia, Campus Mare Nostrum (CMN), 30120, Murcia, Spain; Instituto Murciano de Investigación Biosanitaria Pascual Parrilla (IMIB), Campus de Ciencias de La Salud, 30120, Murcia, Spain. Electronic address: mjoseir@um.es., Chatdarong K; Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand. Electronic address: kaywalee.c@chula.ac.th.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2024 May; Vol. 220, pp. 70-76. Date of Electronic Publication: 2024 Mar 01.
DOI: 10.1016/j.theriogenology.2024.02.032
Abstrakt: Because of the time-consuming nature of surgical neutering and the rapid rate of reproduction among domestic cats, it is crucial to investigate alternative, nonsurgical methods of contraception for this species. Sperm protein IZUMO1 and its oocyte receptor JUNO have been proposed as potential targets for nonsurgical contraceptives. This study aimed to demonstrate (1) the protein coding sequence of feline IZUMO1 and JUNO, (2) gene expression in specific organs by measuring mRNA levels in different visceral tissues, and (3) the expression of IZUMO1 and JUNO during sperm maturation and folliculogenesis, respectively. Amplification for sequencing of feline IZUMO1 and JUNO was performed using the RT-PCR method. Levels of gene expression in different tissues were evaluated using real-time PCR. In situ hybridization was performed to localize JUNO mRNA in ovarian tissues. The complete coding sequences of IZUMO1 and JUNO were obtained and analyzed. A comparison between protein orthologs demonstrated the conservation of IZUMO1 and JUNO in Felidae. The real-time PCR results from various visceral organs indicated that IZUMO1 was significantly higher in the testis than in other organs, whereas JUNO was significantly higher in the ovary than in other organs. Expression of IZUMO1 was found to be higher in the testes than in the caput, corpus, and cauda of epididymides. In situ hybridization revealed that JUNO mRNA was in the ooplasm and nucleus of the primordial, primary, secondary, and antral follicles. Importantly, this was the first study to demonstrate the IZUMO1 and JUNO genes in the testis and ovary of cats. The results are useful for future research related to these genes and for developing contraceptives against these targets.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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