Caatinga, Amazon and Atlantic Forest as natural sources for microbial lignocellulolytic enzymes.

Autor: Ferrari VB; Department of Pharmaceutical Sciences, Universidade Federal de São Paulo (UNIFESP), Diadema, SP, Brazil., Dos Santos Lima LM; Department of Pharmaceutical Sciences, Universidade Federal de São Paulo (UNIFESP), Diadema, SP, Brazil., de Matos Marques K; Department of Pharmaceutical Sciences, Universidade Federal de São Paulo (UNIFESP), Diadema, SP, Brazil., Gutierres FC; Department of Pharmaceutical Sciences, Universidade Federal de São Paulo (UNIFESP), Diadema, SP, Brazil., Guerini GG; Department of Pharmaceutical Sciences, Universidade Federal de São Paulo (UNIFESP), Diadema, SP, Brazil., Silveira MAV; Laboratory of Biochemistry and Enzymology, Institute of Pharmacology and Molecular Biology, Department of Biophysics, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil., de Figueiredo GM; Department of Pharmaceutical Sciences, Universidade Federal de São Paulo (UNIFESP), Diadema, SP, Brazil., Vital VG; Department of Pharmaceutical Sciences, Universidade Federal de São Paulo (UNIFESP), Diadema, SP, Brazil., Roswell MR; Department of Pharmaceutical Sciences, Universidade Federal de São Paulo (UNIFESP), Diadema, SP, Brazil., de Melo IS; Laboratory of Environmental Microbiology, Brazilian Agricultural Research Corporation, EMBRAPA Environment, Jaguariúna, SP, Brazil., Okamoto DN; Department of Pharmaceutical Sciences, Universidade Federal de São Paulo (UNIFESP), Diadema, SP, Brazil., de Vasconcellos SP; Department of Pharmaceutical Sciences, Universidade Federal de São Paulo (UNIFESP), Diadema, SP, Brazil. suzan.pantaroto@unifesp.br.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2024 Mar 14; Vol. 206 (4), pp. 161. Date of Electronic Publication: 2024 Mar 14.
DOI: 10.1007/s00203-024-03883-y
Abstrakt: Brazilian biomes are important sources for environmental microorganisms, including efficient metabolic machineries, like actinomycetes. These bacteria are known for their abilities to produce many bioactive compounds, including enzymes with multiple industrial applications. The present work aimed to evaluate lignocellulolytic abilities of actinomycetes isolated from soil and rhizosphere samples collected at Caatinga, Atlantic and Amazon Forest. Laccase (Lac), lignin peroxidase (LiP), manganese peroxidase (MnP) and cellulase were evaluated for their efficiency. These enzymes have an essential role in lignin decomposition, through oxidation of phenolic and non-phenolic compounds, as well as enzymatic hydrolysis of vegetal biomass. In this sense, a total of 173 actinomycetes were investigated. Eleven (11) of them were selected by their enzymatic performance. The actinomycete AC166 displayed some activity in all analysed scenarios in terms of Lac, MnP and LiP activity, while AC171 was selected as the most promising strain, showing the following activities: 29.7 U.L -1 for Lac; 2.5 U.L -1 for LiP and 23 U.L -1 for MnP. Cellulolytic activities were evaluated at two pH conditions, 4.8 and 7.4, obtaining the following results: 25 U.L -1 and 71 U.L -1 , respectively. Thermostability (4, 30 and 60 o C) and salinity concentrations (0 to 4 M) and pH variation (2.0 to 9.0) stabilities of the obtained LiP and Lac enzymatic extracts were also verified. The actinomycete strain AC171 displayed an adaptable response in distinct pH and salt profiles, indicating that bacterial LiP was some halophilic type. Additionally, the strain AC149 produced an alkali and extreme halophilic lignin peroxidase, which are promising profiles for their future application under lignocellulosic biomass at bioethanol biorefineries.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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