Antihyperglycemic effect of extra virgin sacha inchi oil in type 2 diabetic rats: Mechanisms involved in pancreatic β-cell function and apoptosis.

Autor:	Wongmanee N; Department of Physiology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand., Rojanaverawong W; Department of Physiology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand., Boonsong T; Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand., Hanchang W; Department of Physiology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand.; Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand.
Jazyk:	angličtina
Zdroj:	Journal of traditional and complementary medicine [J Tradit Complement Med] 2023 Aug 24; Vol. 14 (2), pp. 148-161. Date of Electronic Publication: 2023 Aug 24 (Print Publication: 2024).
DOI:	10.1016/j.jtcme.2023.08.005
Abstrakt:	Background and Aim: The purpose of the study was to investigate the anti-hyperglycemic effect of extra virgin sacha inchi oil (EVSIO) and its possible mechanisms and actions against pancreatic β-cell death and dysfunction in type 2 diabetic (T2D) rats. Experimental Procedure: T2D rats were induced with a high-fat diet and low-dose of streptozotocin. The rats were then treated for 5 weeks with EVSIO (0.5, 1, and 2 ml/kg), or pioglitazone. Biochemical and histopathological studies, oxidative and inflammatory markers, and expression of apoptotic-related proteins were then evaluated. Results: EVSIO treatment exhibited a dose-dependent reduction of fasting blood glucose, area under the curve of glucose, total cholesterol, and triglyceride levels in the diabetic rats, while improved pancreatic β-function was demonstrated by increasing pancreatic and serum insulin levels. EVSIO treatment effectively lowered atrophic pancreatic islets and reduced the level of serum and pancreatic MDA in the diabetic rats. In addition to serum and pancreatic GPx activities in the diabetic rats, EVSIO also augmented serum SOD. Increased levels of NF-κB, TNF-α and IL-6 present in the diabetic rats were greatly reduced by EVSIO treatment. Furthermore, EVSIO revealed an anti-apoptotic effect on the diabetic rat pancreas by upregulating Bcl-2, and downregulating Bax and cleaved caspase-3 protein expression. Conclusion: The overall study results demonstrated the potential anti-hyperglycemic effect of EVSIO in the diabetic rats. The beneficial effects of EVSIO may be attributed to its ability to improve pancreatic β-cell function and ameliorate β-cell apoptosis by inhibiting oxidative stress and inflammatory cytokines. Competing Interests: Authors declare that there is no conflict of interest. (© 2023 Center for Food and Biomolecules, National Taiwan University. Production and hosting by Elsevier Taiwan LLC.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu