Immunohistochemistry for Cancer Stem Cell Detection: Principles and Methods.

Autor:	Zannini G; Pathology Unit, Dipartimento di Salute Mentale, Fisica e Medicina Preventiva, Università degli Studi della Campania 'Luigi Vanvitelli', Naples, Italy., Franco R; Pathology Unit, Dipartimento di Salute Mentale, Fisica e Medicina Preventiva, Università degli Studi della Campania 'Luigi Vanvitelli', Naples, Italy. renato.franco@unicampania.it., Zito Marino F; Pathology Unit, Dipartimento di Salute Mentale, Fisica e Medicina Preventiva, Università degli Studi della Campania 'Luigi Vanvitelli', Naples, Italy.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2777, pp. 19-33.
DOI:	10.1007/978-1-0716-3730-2_2
Abstrakt:	Cancer stem cells (CSCs) are rare immortal cells within tumors with capabilities of self-renewal, differentiation, and tumorigenicity. CSCs play a pivotal role in the tumor development, progression, relapse, and resistance of anticancer therapy. The technique of choice to detect CSCs in formalin-fixed and paraffin-embedded (FFPE) samples is immunohistochemistry (IHC) since it is inexpensive and widespread in most laboratories. The main aims of this chapter are the description of the protocols and the automated immunohistochemical systems used for the identification of CSCs. Furthermore, a focus on the most common troubleshooting in CSC IHC is provided. Finally, an overview of the main markers of cancer stem cells in several cancer types will be provided. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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