Recombinant Human Collagen Type III Improves Hypertrophic Scarring by Regulating the Ratio of Type I/III Collagen.

Autor:	Lin-Hui L; Department of Burns, The First Affiliated Hospital of Naval Medical University, Shanghai 200433, China., Yuan-Yuan Z; Department of Burns, The First Affiliated Hospital of Naval Medical University, Shanghai 200433, China., Ming-Yu L; Departmement of Cadres, 967th Hospital of the Joint Logistics Support Force of PLA, Dalian 116000, China., Xu-Dong H; Department of Burns and Plastic, 903rd Hospital of PLA, Hangzhou 313000, China., Yin-Jia D; Department of Burns and Plastic, 903rd Hospital of PLA, Hangzhou 313000, China., Yue Z; Department of Burns and Plastic, 903rd Hospital of PLA, Hangzhou 313000, China., Yang-Hong-Hong F; Department of Burns and Plastic, 903rd Hospital of PLA, Hangzhou 313000, China., Ai-Fen C; Department of Burns and Plastic, 903rd Hospital of PLA, Hangzhou 313000, China., Xu-Dong Z; Department of Burns and Plastic, 903rd Hospital of PLA, Hangzhou 313000, China., Zheng-Li C; Department of Burns, The First Affiliated Hospital of Naval Medical University, Shanghai 200433, China., Jian J; State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200438, China.
Jazyk:	angličtina
Zdroj:	Journal of burn care & research : official publication of the American Burn Association [J Burn Care Res] 2024 Sep 06; Vol. 45 (5), pp. 1269-1273.
DOI:	10.1093/jbcr/irae040
Abstrakt:	Hypertrophic scar development is a complication associated with wound healing, impacting local appearance and function. The type I/III collagen ratio affects the extent of hypertrophic scarring; a reduced ratio can ameliorate this. In this study, recombinant human collagen type III was developed. Liquid chromatography-tandem mass spectrometry was used to determine its amino acid sequence and confirm its high level of homology with natural human type III collagen. Recombinant human collagen type III displayed no cytotoxicity and did not confer skin irritation and sensitization. Immunofluorescence and western blot analyses of histidine following incubation with fibroblasts suggested cell entry of recombinant human collagen type III. Furthermore, recombinant human collagen type III promoted the synthesis of the natural type III collagen in fibroblasts, resulting in a more obvious increase of type III collagen content in fibroblasts than that of type I collagen, and then decreased the ratio of type I/III collagen. The results of 5-ethynyl-2'-deoxyuridine staining assay suggested enhanced fibroblast proliferation. Following local injection of recombinant human collagen type III, rabbit ear scarring was significantly reduced after 60 days. Vancouver Scar Scale evaluation showed that all index scores were significantly reduced. Western blotting and Picro-Sirius red staining showed that the natural type III collagen increase in scar tissue was greater than that of type I collagen, decreasing the type I/III ratio. In summary, recombinant human collagen type III can be taken up by fibroblasts and promote natural collagen synthesis-especially that of type III-thereby reducing the type I/III ratio and improving hypertrophic scarring. (© The Author(s) 2024. Published by Oxford University Press on behalf of the American Burn Association. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)
Databáze:	MEDLINE
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