Evaluation of microbiome in primary and permanent dentition in grade C periodontitis in young individuals.

Autor: Koo SS; Department of Periodontology, School of Dental Medicine, University of Connecticut, Farmington, Connecticut, USA., Fernandes JG; Department of Oral Health Practice, Periodontology Division and Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, Kentucky, USA., Li L; Department of Oral Biology, School of Dental Medicine, University at Buffalo, State University of New York, Buffalo, New York, USA.; UB Microbiome Center, University at Buffalo, State University of New York, Buffalo, New York, USA., Huang H; Department of Periodontology, College of Dentistry, University of Florida, Gainesville, Florida, USA., Aukhil I; Department of Periodontology, College of Dentistry, University of Florida, Gainesville, Florida, USA., Harrison P; Department of Periodontology, College of Dentistry, University of Florida, Gainesville, Florida, USA.; Department of Periodontology, School of Dental Science, Trinity College Dublin, Dublin, Ireland., Diaz PI; Department of Oral Biology, School of Dental Medicine, University at Buffalo, State University of New York, Buffalo, New York, USA.; UB Microbiome Center, University at Buffalo, State University of New York, Buffalo, New York, USA., Shaddox LM; Department of Oral Health Practice, Periodontology Division and Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, Kentucky, USA.; Department of Periodontology, College of Dentistry, University of Florida, Gainesville, Florida, USA.
Jazyk: angličtina
Zdroj: Journal of periodontology [J Periodontol] 2024 Jul; Vol. 95 (7), pp. 650-661. Date of Electronic Publication: 2024 Mar 13.
DOI: 10.1002/JPER.23-0504
Abstrakt: Background: The aim of the present study was to evaluate the subgingival microbiome in patients with grade C molar-incisor pattern periodontitis (C-MIP) affecting the primary or permanent dentitions.
Methods: DNA was isolated from subgingival biofilm samples from diseased and healthy sites from 45 C-MIP patients and subjected to phylogenetic microarray analysis. C-MIP sites were compared between children affected in the primary to those affected in the permanent dentitions. Within-subject differences between C-MIP-affected sites and dentition-matched healthy sites were also evaluated.
Results: C-MIP sites of subjects affected in the primary dentition showed partially overlapping but distinct microbial communities from C-MIP permanent dentition sites (p < 0.05). Differences were due to increased levels in primary C-MIP sites of certain species of the genera Capnocytophaga and Leptotrichia, while C-MIP permanent dentition sites showed higher prevalence of Filifactor alocis. Aggregatibacter actinomycetemcomitans (Aa) was among species seen in high prevalence and levels in both primary and permanent C-MIP sites. Moreover, both permanent and primary C-MIP sites showed distinct microbial communities when compared to dentition-matched healthy sites in the same subject (p < 0.01).
Conclusions: Primary and permanent teeth with C-MIP showed a dysbiotic microbiome, with children affected in the primary dentition showing a distinct profile from those affected in the permanent dentition. However, Aa was enriched in both primary and permanent diseased sites, confirming that this microorganism is implicated in C-MIP in both dentitions.
(© 2024 American Academy of Periodontology.)
Databáze: MEDLINE
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