| Autor: |
Rangsinth P; Department of Pharmacology and Pharmacy, The University of Hong Kong, Hong Kong SAR, China., Pattarachotanant N; Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand., Wang W; Department of Pharmacology and Pharmacy, The University of Hong Kong, Hong Kong SAR, China., Shiu PH; Department of Pharmacology and Pharmacy, The University of Hong Kong, Hong Kong SAR, China., Zheng C; Department of Pharmacology and Pharmacy, The University of Hong Kong, Hong Kong SAR, China., Li R; Department of Pharmacology and Pharmacy, The University of Hong Kong, Hong Kong SAR, China., Tencomnao T; Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand., Chuchawankul S; Department of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand., Prasansuklab A; College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand., Cheung TM; Tian Ran Healthcare Limited, Hong Kong SAR, China., Li J; Department of Rehabilitation Sciences, Faculty of Health and Social Sciences, Hong Kong Polytechnic University, Hong Kong SAR, China., Leung GP; Department of Pharmacology and Pharmacy, The University of Hong Kong, Hong Kong SAR, China. |
| Abstrakt: |
The pharmacological activity and medicinal significance of Amauroderma rugosum (AR) have rarely been documented. We examined the antioxidant and neuroprotective effects of AR on 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in an SH-SY5Y human neuroblastoma cell model of Parkinson's disease (PD) and explored the active ingredients responsible for these effects. The results showed that the AR aqueous extract could scavenge reactive oxygen species and reduce SH-SY5Y cell death induced by 6-OHDA. In addition, the AR aqueous extract increased the survival of Caenorhabditis elegans upon juglone-induced toxicity. Among the constituents of AR, only polysaccharides and gallic acid exhibited antioxidant and neuroprotective effects. The AR aqueous extract reduced apoptosis and increased the expression of phospho-Akt, phospho-mTOR, phospho-MEK, phospho-ERK, and superoxide dismutase-1 in 6-OHDA-treated SH-SY5Y cells. The polysaccharide-rich AR extract was slightly more potent than the aqueous AR extract; however, it did not affect the expression of phospho-Akt or phospho-mTOR. In conclusion, the AR aqueous extract possessed antioxidant and neuroprotective properties against 6-OHDA-induced toxicity in SH-SY5Y cells. The mechanism of action involves the upregulation of the Akt/mTOR and MEK/ERK-dependent pathways. These findings indicate the potential utility of AR and its active ingredients in preventing or treating neurodegenerative disorders associated with oxidative stress such as PD. |
