Secretome from Magnetically Stimulated Muscle Exhibits Anticancer Potency: Novel Preconditioning Methodology Highlighting HTRA1 Action.

Autor: Tai YK; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Institute of Health Technology and Innovation (iHealthtech), National University of Singapore, Singapore 117599, Singapore.; Biolonic Currents Electromagnetic Pulsing Systems Laboratory (BICEPS), National University of Singapore, Singapore 117599, Singapore.; NUS Centre for Cancer Research, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117599, Singapore., Iversen JN; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Institute of Health Technology and Innovation (iHealthtech), National University of Singapore, Singapore 117599, Singapore.; Biolonic Currents Electromagnetic Pulsing Systems Laboratory (BICEPS), National University of Singapore, Singapore 117599, Singapore., Chan KKW; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Institute of Health Technology and Innovation (iHealthtech), National University of Singapore, Singapore 117599, Singapore.; Biolonic Currents Electromagnetic Pulsing Systems Laboratory (BICEPS), National University of Singapore, Singapore 117599, Singapore., Fong CHH; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Institute of Health Technology and Innovation (iHealthtech), National University of Singapore, Singapore 117599, Singapore.; Biolonic Currents Electromagnetic Pulsing Systems Laboratory (BICEPS), National University of Singapore, Singapore 117599, Singapore., Abdul Razar RB; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Institute of Health Technology and Innovation (iHealthtech), National University of Singapore, Singapore 117599, Singapore.; Biolonic Currents Electromagnetic Pulsing Systems Laboratory (BICEPS), National University of Singapore, Singapore 117599, Singapore., Ramanan S; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Institute of Health Technology and Innovation (iHealthtech), National University of Singapore, Singapore 117599, Singapore.; Biolonic Currents Electromagnetic Pulsing Systems Laboratory (BICEPS), National University of Singapore, Singapore 117599, Singapore., Yap LYJ; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Institute of Health Technology and Innovation (iHealthtech), National University of Singapore, Singapore 117599, Singapore.; Biolonic Currents Electromagnetic Pulsing Systems Laboratory (BICEPS), National University of Singapore, Singapore 117599, Singapore., Yin JN; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Institute of Health Technology and Innovation (iHealthtech), National University of Singapore, Singapore 117599, Singapore.; Biolonic Currents Electromagnetic Pulsing Systems Laboratory (BICEPS), National University of Singapore, Singapore 117599, Singapore., Toh SJ; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Institute of Health Technology and Innovation (iHealthtech), National University of Singapore, Singapore 117599, Singapore.; Biolonic Currents Electromagnetic Pulsing Systems Laboratory (BICEPS), National University of Singapore, Singapore 117599, Singapore., Wong CJK; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Institute of Health Technology and Innovation (iHealthtech), National University of Singapore, Singapore 117599, Singapore.; Biolonic Currents Electromagnetic Pulsing Systems Laboratory (BICEPS), National University of Singapore, Singapore 117599, Singapore., Koh PFA; Cancer Science Institute of Singapore, National University of Singapore, Singapore 117599, Singapore., Huang RYJ; Cancer Science Institute of Singapore, National University of Singapore, Singapore 117599, Singapore.; Department of Obstetrics & Gynaecology, National University of Singapore, Singapore 119228, Singapore.; Graduate Institute of Oncology, College of Medicine, National Taiwan University, Taipei 10617, Taiwan.; School of Medicine, College of Medicine, National Taiwan University, Taipei 10617, Taiwan., Franco-Obregón A; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Institute of Health Technology and Innovation (iHealthtech), National University of Singapore, Singapore 117599, Singapore.; Biolonic Currents Electromagnetic Pulsing Systems Laboratory (BICEPS), National University of Singapore, Singapore 117599, Singapore.; NUS Centre for Cancer Research, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117599, Singapore.; Healthy Longevity Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117593, Singapore.; Competence Center for Applied Biotechnology and Molecular Medicine, University of Zürich, 8057 Zürich, Switzerland.
Jazyk: angličtina
Zdroj: Cells [Cells] 2024 Mar 05; Vol. 13 (5). Date of Electronic Publication: 2024 Mar 05.
DOI: 10.3390/cells13050460
Abstrakt: Briefly (10 min) exposing C2C12 myotubes to low amplitude (1.5 mT) pulsed electromagnetic fields (PEMFs) generated a conditioned media (pCM) that was capable of mitigating breast cancer cell growth, migration, and invasiveness in vitro, whereas the conditioned media harvested from unexposed myotubes, representing constitutively released secretome (cCM), was less effective. Administering pCM to breast cancer microtumors engrafted onto the chorioallantoic membrane of chicken eggs reduced tumor volume and vascularity. Blood serum collected from PEMF-exposed or exercised mice allayed breast cancer cell growth, migration, and invasiveness. A secretome preconditioning methodology is presented that accentuates the graded anticancer potencies of both the cCM and pCM harvested from myotubes, demonstrating an adaptive response to pCM administered during early myogenesis that emulated secretome-based exercise adaptations observed in vivo. HTRA1 was shown to be upregulated in pCM and was demonstrated to be necessary and sufficient for the anticancer potency of the pCM; recombinant HTRA1 added to basal media recapitulated the anticancer effects of pCM and antibody-based absorption of HTRA1 from pCM precluded its anticancer effects. Brief and non-invasive PEMF stimulation may represent a method to commandeer the secretome response of muscle, both in vitro and in vivo, for clinical exploitation in breast and other cancers.
Databáze: MEDLINE
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