Plasma Circulating mRNA Profile for the Non-Invasive Diagnosis of Colorectal Cancer Using NanoString Technologies.

Autor: Tsang HF; Department of Clinical Laboratory and Pathology, Hong Kong Adventist Hospital, Hong Kong SAR, China.; Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong SAR, China., Pei XM; Department of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, Hong Kong SAR, China., Wong YKE; Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong SAR, China., Wong SCC; Department of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, Hong Kong SAR, China.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2024 Mar 05; Vol. 25 (5). Date of Electronic Publication: 2024 Mar 05.
DOI: 10.3390/ijms25053012
Abstrakt: Colorectal cancer (CRC) is one of the most prevalent cancers and the second leading cause of cancer deaths in developed countries. Early CRC may have no symptoms and symptoms usually appear with more advanced diseases. Regular screening can identify people who are at increased risk of CRC in order to offer earlier treatment. A cost-effective non-invasive platform for the screening and monitoring of CRC patients allows early detection and appropriate treatment of the disease, and the timely application of adjuvant therapy after surgical operation is needed. In this study, a cohort of 71 plasma samples that include 48 colonoscopy- and histopathology-confirmed CRC patients with TNM stages I to IV were recruited between 2017 and 2019. Plasma mRNA profiling was performed in CRC patients using NanoString nCounter. Normalized data were analyzed using a Mann-Whitney U test to determine statistically significant differences between samples from CRC patients and healthy subjects. A multiple-group comparison of clinical phenotypes was performed using the Kruskal-Wallis H test for statistically significant differences between multiple groups. Among the 27 selected circulating mRNA markers, all of them were found to be overexpressed (gene expression fold change > 2) in the plasma of patients from two or more CRC stages. In conclusion, NanoString-based targeted plasma CRC-associated mRNAs circulating the marker panel that can significantly distinguish CRC patients from a healthy population were developed for the non-invasive diagnosis of CRC using peripheral blood samples.
Databáze: MEDLINE
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