| Autor: |
Iemmolo M; Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, 90123 Palermo, Italy., Bivona G; Department of Biomedicine Neurosciences and Advanced Diagnostics, University of Palermo, 90127 Palermo, Italy., Piccoli T; Department of Laboratory Medicine, University Hospital 'P. Giaccone', 90127 Palermo, Italy., Nicosia A; Institute for Biomedical Research and Innovation-National Research Council (IRIB-CNR), 90146 Palermo, Italy., Schiera G; Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, 90123 Palermo, Italy., Di Liegro CM; Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, 90123 Palermo, Italy., Di Pietra F; Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, 90123 Palermo, Italy., Ghersi G; Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, 90123 Palermo, Italy. |
| Abstrakt: |
Alzheimer's disease (AD) is the most common form of dementia, characterized by the accumulation of β-amyloid plaques, tau tangles, neuroinflammation, and synaptic/neuronal loss, the latter being the strongest correlating factor with memory and cognitive impairment. Through an in vitro study on a neurons-astrocytes-microglia (NAM) co-culture system, we analyzed the effects of cerebrospinal fluid (CSF) samples from AD and non-AD patients (other neurodegenerative pathologies). Treatment with CSF from AD patients showed a loss of neurofilaments and spheroids, suggesting the presence of elements including CX3CL1 (soluble form), destabilizing the neurofilaments, cellular adhesion processes, and intercellular contacts. The NAM co-cultures were analyzed in immunofluorescence assays for several markers related to AD, such as through zymography, where the expression of proteolytic enzymes was quantified both in cell extracts and the co-cultures' conditioned medium (CM). Through qRT-PCR assays, several genes involved in the formation of β-amyloid plaque, in phosphorylation of tau, and in inflammation pathways and MMP expression were investigated. |
