| Autor: |
Shira KA; Animal, Veterinary, and Food Science Department, University of Idaho, Moscow, ID 83843, USA., Murdoch BM; Animal, Veterinary, and Food Science Department, University of Idaho, Moscow, ID 83843, USA., Thornton KJ; Department of Animal, Dairy and Veterinary Science, Utah State University, 4815 Old Main Hill, Logan, UT 84322, USA., Reichhardt CC; Department of Human Nutrition, Food and Animal Sciences, University of Hawai'i at Manoa, 1955 East-West Rd., Honolulu, HI 96822, USA., Becker GM; Animal, Veterinary, and Food Science Department, University of Idaho, Moscow, ID 83843, USA., Chibisa GE; Animal, Veterinary, and Food Science Department, University of Idaho, Moscow, ID 83843, USA., Murdoch GK; Animal, Veterinary, and Food Science Department, University of Idaho, Moscow, ID 83843, USA.; Department of Animal Sciences, Washington State University, Pullman, WA 99163, USA. |
| Abstrakt: |
The myokines interleukin 6 (IL-6), interleukin 15 (IL-15), myonectin (CTRP15), fibronectin type III domain containing protein 5/irisin (FNDC5), and brain-derived neurotrophic factor (BDNF) are associated with skeletal muscle cell proliferation, differentiation, and muscle hypertrophy in biomedical model species. This study evaluated whether these myokines are produced by cultured bovine satellite cells (BSCs) harvested from 3- and 11-month-old commercial black Angus steers and if the expression and secretion of these targets change across 0, 12, 24, and 48 h in vitro. IL-6 , IL-15, FNDC5 , and BDNF expression were greater ( p ≤ 0.05) in the differentiated vs. undifferentiated BSCs at 0, 12, 24, and 48 h. CTRP15 expression was greater ( p ≤ 0.03) in the undifferentiated vs. differentiated BSCs at 24 and 48 h. IL-6 and CTRP15 protein from culture media were greater ( p ≤ 0.04) in undifferentiated vs. differentiated BSCs at 0, 12, 24, and 48 h. BDNF protein was greater in the media of differentiated vs. undifferentiated BSCs at 0, 12, 24, and 48 h. IL-6 , 1L-15 , FNDC5 , and BDNF are expressed in association with BSC differentiation, and CTRP15 appears to be expressed in association with BSC proliferation. This study also confirms IL-6, IL-15, CTRP15, and BDNF proteins present in media collected from primary cultures of BSCs. |
