Hydrogels Based on Recombinant Spidroin Stimulate Proliferation and Migration of Human Corneal Cells.

Autor: Agapova OI; Academician Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Moscow, Russia., Ostrovsky DS; Fyodorov Eye Microsurgery Complex, Ministry of Health of the Russian Federation, Moscow, Russia., Khubetsova MK; Fyodorov Eye Microsurgery Complex, Ministry of Health of the Russian Federation, Moscow, Russia., Kerimov TZ; Fyodorov Eye Microsurgery Complex, Ministry of Health of the Russian Federation, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Ministry of Health of the Russian Federation, Moscow, Russia., Borzenok SA; Fyodorov Eye Microsurgery Complex, Ministry of Health of the Russian Federation, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Ministry of Health of the Russian Federation, Moscow, Russia., Bogush VG; Kurchatov Institute National Research Center, Moscow, Russia., Davydova LI; Kurchatov Institute National Research Center, Moscow, Russia., Cheperegin SE; Kurchatov Institute National Research Center, Moscow, Russia., Efimov AE; Academician Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Moscow, Russia., Agapov II; Academician Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Moscow, Russia. igor_agapov@gmail.com., Debabov VG; Kurchatov Institute National Research Center, Moscow, Russia.
Jazyk: angličtina
Zdroj: Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections [Dokl Biol Sci] 2023 Dec; Vol. 513 (Suppl 1), pp. S41-S44. Date of Electronic Publication: 2024 Mar 12.
DOI: 10.1134/S0012496623600173
Abstrakt: The effect of recombinant spidroin (RS) hydrogel (HG) on anterior epithelial cells and keratocytes of the human cornea was studied in vitro. Corneal injuries are highly prevalent in developing countries according to the World Health Organization. Various technologies have recently been proposed to restore the damaged surface of the cornea. Use of biodegradable silk-based materials, including recombinant analogs of the spider silk protein spidroin, is an important avenue of research in the field of wound healing and corneal regeneration. Spidroins are well known for their optimal balance of strength and elasticity. Given their biological compatibility, lack of immunogenicity, and biodegradability, spidroins provide a biomaterial for tissue engineering and regenerative medicine. HGs based on RS rS2/12-RGDS were therefore tested for cytotoxicity toward isolated corneal epithelial cells and keratocytes with regard to possible changes in cell phenotype and migratory activity. A promising outlook and therapeutic potential were demonstrated for RS-based HGs.
(© 2023. Pleiades Publishing, Ltd.)
Databáze: MEDLINE
Externí odkaz: