A smartphone-integrated aptasensor for pesticide detection using gold-decorated microparticles.

Autor: Ulloa-Gomez AM; School of Materials Engineering, Purdue University, West Lafayette, IN, 47907, USA., Waimin JF; School of Materials Engineering, Purdue University, West Lafayette, IN, 47907, USA., Yu YC; School of Materials Engineering, Purdue University, West Lafayette, IN, 47907, USA., Lucas A; School of Materials Engineering, Purdue University, West Lafayette, IN, 47907, USA., Stanciu LA; School of Materials Engineering, Purdue University, West Lafayette, IN, 47907, USA. lstanciu@purdue.edu.; Bindley Bioscience Center, Purdue University, West Lafayette, IN, 47907, USA. lstanciu@purdue.edu.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 Mar 12; Vol. 191 (4), pp. 194. Date of Electronic Publication: 2024 Mar 12.
DOI: 10.1007/s00604-024-06255-x
Abstrakt: The increasing incidence of environmental concerns related to excessive use of pesticides, such as imidacloprid and carbendazim, poses risks to pollinators, water bodies, and human health, prompting regulatory scrutiny and bans in developed countries. In this study, we propose a portable smartphone-based biosensor for rapid and label-free colorimetric detection by using the gold-decorated polystyrene microparticles (Ps-AuNP) functionalized with specific aptamers to imidacloprid and carbendazim on a microfluidic paper-based analytical device (μ-PAD). Four aptamers were selected for the detection of these pesticides and their sensitivity and selectivity performance was evaluated. The sensitivity results show a detection limit for imidacloprid of 3.12 ppm and 1.56 ppm for carbendazim. The aptamers also exhibited high selectivity performance against other pesticides, such as thiamethoxam, fenamiphos, isoproturon, and atrazine. However, the platform presented cross-selectivity when detecting imidacloprid, carbendazim, and linuron, which is discussed herein. Overall, we present a promising platform for simple, on-site, and rapid colorimetric screening of specific pesticides, while highlighting the challenges of aptasensors in achieving selectivity amidst diverse molecular structures.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: