Identification of two mutant JASON-RELATED genes associated with unreduced pollen production in potato.

Autor: Clot CR; Plant Breeding, Wageningen University and Research, Po Box 386, 6700 AJ, Wageningen, The Netherlands., Vexler L; Plant Breeding, Wageningen University and Research, Po Box 386, 6700 AJ, Wageningen, The Netherlands.; Teagasc, Crops Research, Oak Park, Carlow, R93 XE12, Ireland., de La O Leyva-Perez M; Teagasc, Crops Research, Oak Park, Carlow, R93 XE12, Ireland., Bourke PM; Plant Breeding, Wageningen University and Research, Po Box 386, 6700 AJ, Wageningen, The Netherlands., Engelen CJM; Plant Breeding, Wageningen University and Research, Po Box 386, 6700 AJ, Wageningen, The Netherlands., Hutten RCB; Plant Breeding, Wageningen University and Research, Po Box 386, 6700 AJ, Wageningen, The Netherlands., van de Belt J; Laboratory of Genetics, Wageningen University and Research, Po Box 16, 6700 AA, Wageningen, The Netherlands., Wijnker E; Laboratory of Genetics, Wageningen University and Research, Po Box 16, 6700 AA, Wageningen, The Netherlands., Milbourne D; Teagasc, Crops Research, Oak Park, Carlow, R93 XE12, Ireland., Visser RGF; Plant Breeding, Wageningen University and Research, Po Box 386, 6700 AJ, Wageningen, The Netherlands., Juranić M; Plant Breeding, Wageningen University and Research, Po Box 386, 6700 AJ, Wageningen, The Netherlands., van Eck HJ; Plant Breeding, Wageningen University and Research, Po Box 386, 6700 AJ, Wageningen, The Netherlands. herman.vaneck@wur.nl.
Jazyk: angličtina
Zdroj: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2024 Mar 12; Vol. 137 (4), pp. 79. Date of Electronic Publication: 2024 Mar 12.
DOI: 10.1007/s00122-024-04563-7
Abstrakt: Key Message: Multiple QTLs control unreduced pollen production in potato. Two major-effect QTLs co-locate with mutant alleles of genes with homology to AtJAS, a known regulator of meiotic spindle orientation. In diploid potato the production of unreduced gametes with a diploid (2n) rather than a haploid (n) number of chromosomes has been widely reported. Besides their evolutionary important role in sexual polyploidisation, unreduced gametes also have a practical value for potato breeding as a bridge between diploid and tetraploid germplasm. Although early articles argued for a monogenic recessive inheritance, the genetic basis of unreduced pollen production in potato has remained elusive. Here, three diploid full-sib populations were genotyped with an amplicon sequencing approach and phenotyped for unreduced pollen production across two growing seasons. We identified two minor-effect and three major-effect QTLs regulating this trait. The two QTLs with the largest effect displayed a recessive inheritance and an additive interaction. Both QTLs co-localised with genes encoding for putative AtJAS homologs, a key regulator of meiosis II spindle orientation in Arabidopsis thaliana. The function of these candidate genes is consistent with the cytological phenotype of mis-oriented metaphase II plates observed in the parental clones. The alleles associated with elevated levels of unreduced pollen showed deleterious mutation events: an exonic transposon insert causing a premature stop, and an amino acid change within a highly conserved domain. Taken together, our findings shed light on the natural variation underlying unreduced pollen production in potato and will facilitate interploidy breeding by enabling marker-assisted selection for this trait.
(© 2024. The Author(s).)
Databáze: MEDLINE
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