One Health approach for Brucella canis: Serological and molecular detection in animal-hoarding individuals and their dogs.

Autor: Schiavo L; Graduate College of Cell and Molecular Biology, Federal University of Paraná (UFPR), Curitiba, Paraná, Brazil.; Paraná State Technology Institute (Tecpar), Paraná State Government, Curitiba, Paraná, Brazil., Ribeiro ML; Department of Animal Production and Preventive Veterinary Medicine, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil., de Almeida MB; Paraná State Technology Institute (Tecpar), Paraná State Government, Curitiba, Paraná, Brazil., da Cunha GR; Secretary of the Environment, City Hall of Pinhais, Pinhais, Paraná, Brazil., Espírito Santo GAN; Paraná State Technology Institute (Tecpar), Paraná State Government, Curitiba, Paraná, Brazil., Morikawa VM; Secretary of the Environment, City Hall of Curitiba, Curitiba, Paraná, Brazil., Vicente AF; WOAH/EU & National Reference Laboratory for Animal Brucellosis, Animal Health Laboratory, Paris-Est University/Anses, Paris, France., Ponsart C; WOAH/EU & National Reference Laboratory for Animal Brucellosis, Animal Health Laboratory, Paris-Est University/Anses, Paris, France., de Santi CE; Department of Veterinary Medicine, Federal University of Paraná (UFPR), Curitiba, Paraná, Brazil., Kmetiuk LB; Zoonosis Surveillance Unit, City Secretary of Health, Curitiba, Paraná, Brazil., Megid J; Department of Animal Production and Preventive Veterinary Medicine, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil., Biondo AW; Graduate College of Cell and Molecular Biology, Federal University of Paraná (UFPR), Curitiba, Paraná, Brazil.; Department of Veterinary Medicine, Federal University of Paraná (UFPR), Curitiba, Paraná, Brazil.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2024 Mar 12; Vol. 18 (3), pp. e0011974. Date of Electronic Publication: 2024 Mar 12 (Print Publication: 2024).
DOI: 10.1371/journal.pntd.0011974
Abstrakt: Animal hoarding disorder (AHD) is classified as a psychiatric obsessive-compulsive condition characterized by animal accumulation and often accompanied by unsanitary conditions and animal cruelty. Although AHD may increase pathogen transmission and spread, particularly for zoonotic diseases, human and dog exposure in such cases has yet to be fully established. Accordingly, this study aimed to assess Brucella canis in 19 individuals with AHD (11 households) and their 264 dogs (21 households) in Curitiba, the eighth largest city in Brazil, with approximately 1.8 million habitants. Anti-B. canis antibodies were detected by the 2-mercaptoethanol microplate agglutination test (2ME-MAT) and by a commercial lateral flow immunoassay (LFIA), while molecular detection of previously positive seropositive samples was performed by conventional PCR. Although all the human samples were 2ME-MAT negative, 12/264 (4.5%, 95% Confidence Interval: 2.0-7.0%) dog samples were 2ME-MAT and LFIA positive, with 2ME-MAT titers ranging from 20 to 640. At least one dog in 4/21 (19.0%, 95% CI: 2.0-46.0%) households was seropositive. Despite the absence of seropositivity in individuals with AHD and the comparatively low seroprevalence in dogs, B. canis circulation and outbreaks should be considered in such human populations due to the high burden and recurrent character of B. canis exposure in high-density dog populations and the constant introduction of susceptible animals.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Schiavo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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