| Autor: |
Chen H; Guangdong Provincial Key Laboratory of Food Quality and Safety/National-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 510642, China. liuyunle@scau.edu.cn., Ye L; Guangdong Provincial Key Laboratory of Food Quality and Safety/National-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 510642, China. liuyunle@scau.edu.cn., Wang Y; Guangdong Provincial Key Laboratory of Food Quality and Safety/National-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 510642, China. liuyunle@scau.edu.cn., Chen J; Guangdong Provincial Key Laboratory of Food Quality and Safety/National-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 510642, China. liuyunle@scau.edu.cn., Wang J; Guangdong Provincial Key Laboratory of Food Quality and Safety/National-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 510642, China. liuyunle@scau.edu.cn., Li X; Guangdong Provincial Key Laboratory of Food Quality and Safety/National-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 510642, China. liuyunle@scau.edu.cn., Lei H; Guangdong Provincial Key Laboratory of Food Quality and Safety/National-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 510642, China. liuyunle@scau.edu.cn.; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China.; Heyuan Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Heyuan, 517000, China., Liu Y; Guangdong Provincial Key Laboratory of Food Quality and Safety/National-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 510642, China. liuyunle@scau.edu.cn.; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China.; Heyuan Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Heyuan, 517000, China. |
| Abstrakt: |
Aflatoxin B 1 (AFB 1 ) causes serious immunotoxicity and has attracted considerable attention owing to its high sensitivity and common chemical-viral interactions in living organisms. However, the sensitivity of different species to AFB 1 widely varies, which cannot be explained by the different metabolism in species. The gut microbiota plays a crucial role in the immune system, but the interaction of the microbiota with AFB 1 -induced immunotoxicity still needs to be determined. Our results indicated that AFB 1 exposure disrupted the structure of the gut microbiota and damaged the gut barrier, which caused translocation of microbiota metabolites, lipopolysaccharides, to the spleen. Subsequently, pyroptosis of the spleen was activated. Interestingly, AFB 1 exposure had little effect on the splenic pyroptosis of pseudo-germfree mice (antibiotic mixtures eliminated their gut microbiota, ABX). Then, fecal microbiota transplant (FMT) and sterile fecal filtrate (SFF) were employed to validate the function of the gut microbiota and its metabolites in AFB 1 -induced splenic pyroptosis. The AFB 1 -disrupted microbiota and its metabolites significantly promoted splenic pyroptosis, which was worse than that in control mice. Overall, AFB 1 -induced splenic pyroptosis is associated with the gut microbiota and its metabolites, which was further demonstrated by FMT and SFF. The mechanism of AFB 1 -induced splenic pyroptosis was explored for the first time, which paves a new way for preventing and treating the immunotoxicity from mycotoxins by regulating the gut microbiota. |
