Micro/nanofluidic device for tamsulosin therapeutic drug monitoring in patients with benign prostatic hyperplasia at point of care.

Autor: Al-Aqbi ZT; Department of Chemistry, Faculty of Science, University of Basrah, Basrah, 61004, Iraq. zthal@utas.edu.au.; Department of Chemistry, College of Science, University of Misan, Maysan, 62001, Iraq. zthal@utas.edu.au., Abdulsahib HT; Department of Chemistry, Faculty of Science, University of Basrah, Basrah, 61004, Iraq., Al-Doghachi FAJ; Department of Chemistry, Faculty of Science, University of Basrah, Basrah, 61004, Iraq.
Jazyk: angličtina
Zdroj: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2024 Jun; Vol. 40 (6), pp. 1101-1110. Date of Electronic Publication: 2024 Mar 11.
DOI: 10.1007/s44211-024-00533-7
Abstrakt: Discovering the balance between toxicity and efficacy for many drugs requires therapeutic drug monitoring (TDM) of their concentrations in the blood. Here, a hot-embossed microfluidic device with a new design integrated to a nanofracture is presented for purification of blood samples from numerous proteins and cells, allowing to the separation of small molecules from blood matrix. The device was used to separate and quantitatively detect tamsulosin drug after derivatization with fluorescamine reagent, allowing converting it from a neutral molecule into a charged fluorescent complex under the experimental conditions, and thus its separation by electrophoresis. The device is portable and easy operated, and the presented method showed good linearity (R 2  = 0.9948) over a concentration range of 0.1-1 μg/mL. The relative standard deviation (RSD%) was below 10% (n = 3), indicating good precisions, and the limit of detection (LOD) and limit of quantitation (LOQ) values were estimated to be 0.1 and 0.55 μg/mL, respectively. Whole blood samples from 10 patients with benign prostatic hyperplasia (BPH) were analyzed, showing good percentage recoveries of tamsulosin in whole blood. This point-of-care (POC), low-cost method could increase the convenience of patients and doctors, make therapies safer, and make TDM available in different regions and places.
(© 2024. The Author(s), under exclusive licence to The Japan Society for Analytical Chemistry.)
Databáze: MEDLINE
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