Multimodal Control of Bacterial Gene Expression by Red and Blue Light.

Autor:	Meier SSM; Department of Biochemistry, University of Bayreuth, Bayreuth, Germany., Multamäki E; Department of Anatomy, University of Helsinki, Helsinki, Finland., Ranzani AT; Department of Biochemistry, University of Bayreuth, Bayreuth, Germany., Takala H; Department of Anatomy, University of Helsinki, Helsinki, Finland.; Department of Biological and Environmental Science, Nanoscience Center, University of Jyvaskyla, Jyvaskyla, Finland., Möglich A; Department of Biochemistry, University of Bayreuth, Bayreuth, Germany. andreas.moeglich@uni-bayreuth.de.; Bayreuth Center for Biochemistry & Molecular Biology, Universität Bayreuth, Bayreuth, Germany. andreas.moeglich@uni-bayreuth.de.; North-Bavarian NMR Center, Universität Bayreuth, Bayreuth, Germany. andreas.moeglich@uni-bayreuth.de.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2760, pp. 463-477.
DOI:	10.1007/978-1-0716-3658-9_26
Abstrakt:	By applying sensory photoreceptors, optogenetics realizes the light-dependent control of cellular events and state. Given reversibility, noninvasiveness, and exquisite spatiotemporal precision, optogenetic approaches enable innovative use cases in cell biology, synthetic biology, and biotechnology. In this chapter, we detail the implementation of the pREDusk, pREDawn, pCrepusculo, and pAurora optogenetic circuits for controlling bacterial gene expression by red and blue light, respectively. The protocols provided here guide the practical use and multiplexing of these circuits, thereby enabling graded protein production in bacteria at analytical and semi-preparative scales. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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