Effects of moxibustion at "Zusanli" (ST 36) on oxidative stress and intestinal flora in aging rats.

Autor:	Zhao C; College of Acupuncture-Moxibustion and Tuina, Guangxi University of CM, Nanning 530001, China. zhcjiao@126.com., Chen S; College of Acupuncture-Moxibustion and Tuina, Guangxi University of CM, Nanning 530001, China., Li X; College of Acupuncture-Moxibustion and Tuina, Guangxi University of CM, Nanning 530001, China., Miao F; College of Acupuncture-Moxibustion and Tuina, Guangxi University of CM, Nanning 530001, China., Zeng N; College of Acupuncture-Moxibustion and Tuina, Guangxi University of CM, Nanning 530001, China., Kuang Y; College of Acupuncture-Moxibustion and Tuina, Guangxi University of CM, Nanning 530001, China.
Jazyk:	English; Chinese
Zdroj:	Zhongguo zhen jiu = Chinese acupuncture & moxibustion [Zhongguo Zhen Jiu] 2024 Mar 12; Vol. 44 (3), pp. 303-308.
DOI:	10.13703/j.0255-2930.20221016-k0004
Abstrakt:	Objectives: To observe the effects of moxibustion at "Zusanli"(ST 36)on oxidative stress and intestinal flora in subacute aging rats, and to explore the possible mechanism of moxibustion in delaying aging. Methods: Thirty SD rats were randomly divided into a blank group, a model group and a Zusanli group, with 10 rats in each group. Subacute aging model was established by intraperitoneal injection of D-galactose at dosage of 500 mg/kg in the model group and the Zusanli group, once a day for 42 days. In the Zusanli group, moxibustion was applied at bilateral "Zusanli" (ST 36) , once a day, 3 moxa cones at each acupoint, for consecutive 28 days. After intervention, the serum levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were detected by ELISA; the intestinal flora was detected by 16S rRNA sequencing technique in each group. Results: Compared with the blank group, the serum level of SOD was decreased ( P <0.01), the serum level of MDA was increased ( P <0.01) in the model group. Compared with the model group, the serum level of SOD was increased ( P <0.01), the serum level of MDA was decreased ( P <0.01) in the Zusanli group. Compared with the blank group, Chao1 and Shannon indexes were decreased in the model group ( P <0.01, P <0.05). Compared with the model group, Chao1 and Shannon indexes were increased in the Zusanli group ( P <0.01, P <0.05). Compared with the blank group, the relative abundance of Firmicutes , Treponema_ 2 and Lachnospiraceae_ NK4A136_group was increased ( P <0.05, P <0.01), while the relative abundance of Prevotellaceae_ UCG-003 and the relative abundance ratio of Bacteroidetes and Firmicutes (B/F value) were decreased ( P <0.01, P <0.05) in the model group. Compared with the model group, the relative abundance of Firmicutes and Treponema_ 2 was decreased ( P <0.01), while the relative abundance of Bacteroidetes , Lactobacillus , Prevotellaceae_ UCG-003 and B/F value were increased ( P <0.05, P <0.01) in the Zusanli group. Conclusions: Moxibustion at "Zusanli"(ST 36)can effectively improve the level of oxidative stress, regulate the constitution of intestinal flora, maintain the microecological balance of intestinal flora in aging rats, and thus play a role in delaying aging.
Databáze:	MEDLINE
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