Towards phenotyping adaptive traits in camels: A study of the influence of hypotonic saline solutions on blood cell area.

Autor: Alhaddad H; Department of Biological Sciences, Faculty of Science, Kuwait University, Shadadiya, Kuwait., Alnughaimish A; Department of Biological Sciences, Faculty of Science, Kuwait University, Shadadiya, Kuwait., Alhajeri D; Department of Biological Sciences, Faculty of Science, Kuwait University, Shadadiya, Kuwait., Alhajeri BH; Department of Biological Sciences, Faculty of Science, Kuwait University, Shadadiya, Kuwait.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Mar 11; Vol. 19 (3), pp. e0298336. Date of Electronic Publication: 2024 Mar 11 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0298336
Abstrakt: Single-humped camels are livestock of physical, physiological, and biochemical adaptations to hot desert environments and to water scarcity. The tolerance of camels to water deprivation and their exceptional capacity for rapid rehydration requires blood cells with membranes of specialized organization and chemical composition. The objectives of this study are to examine the changes in the area (a proxy for volume) of camel blood cells in solutions with decreasing concentrations of NaCl and consequently identify the conditions under which blood cells can be phenotyped in a large population. Whole-blood samples from three healthy adult female camels were treated with four different concentrations of NaCl and examined at six incubation-periods. Observationally, red blood cells in all treatments remained intact and maintained their elliptical shape while white blood cells experienced some damage, lysing at concentrations below 0.90%. Average basal (in 0.90% NaCl) RBC area was ~15 μm² and swelled in the various treatments, in some cases reaching twice its original size. Excluding the damaged cells, the average area of combined WBCs, ~32.7 μm², expanded approximately three times its original size. We find that camel WBCs, like their RBCs, are adapted to hypotonic environments, and are capable of expanding while maintaining their structural integrity.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Alhaddad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje