Autor: |
Shen SA; Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins School of Medicine; sarek.shen@gmail.com., Goyal MM; Department of Chemical and Biomolecular Engineering, Johns Hopkins Whiting School of Engineering., Lane K; Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins School of Medicine., Lehar M; Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins School of Medicine., Sun DQ; Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins School of Medicine; Department of Otolaryngology-Head and Neck Surgery, University of Cincinnati College of Medicine. |
Jazyk: |
angličtina |
Zdroj: |
Journal of visualized experiments : JoVE [J Vis Exp] 2024 Feb 23 (204). Date of Electronic Publication: 2024 Feb 23. |
DOI: |
10.3791/65816 |
Abstrakt: |
Efficient and minimally invasive drug delivery to the inner ear is a significant challenge. The round window membrane (RWM), being one of the few entry points to the inner ear, has become a vital focus of investigation. However, due to the complexities of isolating the RWM, our understanding of its pharmacokinetics remains limited. The RWM comprises three distinct layers: the outer epithelium, the middle connective tissue layer, and the inner epithelial layer, each potentially possessing unique delivery properties. Current models for investigating transport across the RWM utilize in vivo animal models or ex vivo RWM models which rely on cell cultures or membrane fragments. Guinea pigs serve as a validated preclinical model for the investigation of drug pharmacokinetics within the inner ear and are an important animal model for the translational development of delivery vehicles to the cochlea. In this study, we describe an approach for explantation of a guinea pig RWM with surrounding cochlear bone for benchtop drug delivery experiments. This method allows for preservation of native RWM architecture and may provide a more realistic representation of barriers to transport than current benchtop models. |
Databáze: |
MEDLINE |
Externí odkaz: |
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