The Mayo Clinic Salivary Tissue-Organoid Biobanking: A Resource for Salivary Regeneration Research.
| Autor: | Aalam SMM; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA., Varela AR; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA., Khaderi A; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA., Mondesir RJ; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA., Mun DG; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA., Ding A; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA., Lombaert IMA; Biologic and Materials Sciences and Prosthodontics, University of Michigan School of Dentistry, 1011 N. University Ave, Ann Arbor, MI, USA.; Biointerfaces Institute, University of Michigan, 2900 Plymouth Rd, Ann Arbor, MI, USA., Coppes RP; Departments of Radiation Oncology and Biomedical Sciences, Section Molecular Cell Biology, University of Groningen, University Medical Center Groningen, The Netherlands., Emperumal CP; Department of Otolaryngology-Head and Neck Surgery, Mayo Clinic, Rochester, MN, USA., Pandey A; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.; Center for Individualized Medicine, Mayo Clinic, Rochester, MN, USA.; Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India., Janus JR; Department of Otolaryngology-Head and Neck Surgery, Mayo Clinic, Jacksonville, FL, USA.; Center for Regenerative Biotherapeutics, Mayo Clinic, Rochester, MN, USA., Kannan N; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.; Center for Regenerative Biotherapeutics, Mayo Clinic, Rochester, MN, USA.; Mayo Clinic Comprehensive Cancer Center, Mayo Clinic, Rochester, MN, USA. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | BioRxiv : the preprint server for biology [bioRxiv] 2024 Feb 27. Date of Electronic Publication: 2024 Feb 27. |
| DOI: | 10.1101/2024.02.23.581761 |
| Abstrakt: | The salivary gland (SG) is an essential organ that secretes saliva, which supports versatile oral function throughout life, and is maintained by elusive epithelial stem and progenitor cells (SGSPC). Unfortunately, aging, drugs, autoimmune disorders, and cancer treatments can lead to salivary dysfunction and associated health consequences. Despite many ongoing therapeutic efforts to mediate those conditions, investigating human SGSPC is challenging due to lack of standardized tissue collection, limited tissue access, and inadequate purification methods. Herein, we established a diverse and clinically annotated salivary regenerative biobanking at the Mayo Clinic, optimizing viable salivary cell isolation and clonal assays in both 2D and 3D-matrigel growth environments. Our analysis identified ductal epithelial cells in vitro enriched with SGSPC expressing the CD24/EpCAM/CD49f+ and PSMA- phenotype. We identified PSMA expression as a reliable SGSPC differentiation marker. Moreover, we identified progenitor cell types with shared phenotypes exhibiting three distinct clonal patterns of salivary differentiation in a 2D environment. Leveraging innovative label-free unbiased LC-MS/MS-based single-cell proteomics, we identified 819 proteins across 71 single cell proteome datasets from purified progenitor-enriched parotid gland (PG) and sub-mandibular gland (SMG) cultures. We identified distinctive co-expression of proteins, such as KRT1/5/13/14/15/17/23/76 and 79, exclusively observed in rare, scattered salivary ductal basal cells, indicating the potential de novo source of SGSPC. We also identified an entire class of peroxiredoxin peroxidases, enriched in PG than SMG, and attendant H Competing Interests: Conflict of Interest statement: All the co-authors declare that there is no conflict of interest in relation to the work described. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|