Initial acidic media promotes quiescence entry in Saccharomyces cerevisiae .
| Autor: | Greenlaw A; Basic Sciences Division, Fred Hutch Cancer Center, Seattle, Washington, United States., Dell R; Basic Sciences Division, Fred Hutch Cancer Center, Seattle, Washington, United States., Tsukiyama T; Basic Sciences Division, Fred Hutch Cancer Center, Seattle, Washington, United States. |
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| Jazyk: | angličtina |
| Zdroj: | MicroPublication biology [MicroPubl Biol] 2024 Feb 24; Vol. 2024. Date of Electronic Publication: 2024 Feb 24 (Print Publication: 2024). |
| DOI: | 10.17912/micropub.biology.001071 |
| Abstrakt: | Quiescence is a conserved cellular state wherein cells cease proliferation and remain poised to re-enter the cell cycle when conditions are appropriate. Budding yeast is a powerful model for studying cellular quiescence. In this work, we demonstrate that the pH of the YPD media strongly affects quiescence entry efficiency in Saccharomyces cerevisiae. Adjusting the initial media pH to 5.5 significantly improves quiescence entry efficiency compared to unadjusted YPD media. Thermotolerance of the produced quiescence yeast are similar, suggesting the media pH influences the quantity of quiescent cells more than quality of quiescence reached. Competing Interests: The authors declare that there are no conflicts of interest present. (Copyright: © 2024 by the authors.) |
| Databáze: | MEDLINE |
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