Efficient caproic acid production from lignocellulosic biomass by bio-augmented mixed microorganisms.

Autor: Liu T; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China., Li J; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China., Hao X; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China., Meng J; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address: jiameng@hit.edu.cn.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2024 May; Vol. 399, pp. 130565. Date of Electronic Publication: 2024 Mar 08.
DOI: 10.1016/j.biortech.2024.130565
Abstrakt: Producing caproic acid via carboxylate platform is an environmentally-friendly approach for treating lignocellulosic agricultural waste. However, its implementation is still challenged by low product yields and selectivity. A microbiome named cellulolytic acid-producing microbiome (DCB), proficient in producing cellulolytic acid, was successfully acquired and shows promise for producing high-level caproic acid. In this study, a bioaugmentation method utilizing Clostridium kluyveri is proposed to enhance caproic acid yield of DCB using rice straw. With exogenous ethanol, bioaugmentation with Clostridium kluyveri significantly improved the caproic acid concentration and selectivity by 7 times and 4.5 times, achieving 12.9 g/L and 55.1 %, respectively. The addition of Clostridium kluyveri introduced reverse β-oxidation pathway, a more efficient caproic acid production pathway. Meanwhile, bioaugmentation enriched the bacteria proficient in degrading straw and producing short-chain fatty acids, providing more substrates for caproic acid production. This study provides potential bioaugmentation strategies for optimizing caproic acid yield from lignocellulosic biomass.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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