The essential oil of Melaleuca alternifolia incorporated into hydrogel induces antimicrobial and anti-inflammatory effects on infected wounds by Staphylococcus aureus.

Autor: Vila Nova BG; Microbial Pathogenicity Laboratory, CEUMA University, São Luís, MA, Brazil., Silva LDS; Microbial Pathogenicity Laboratory, CEUMA University, São Luís, MA, Brazil., Andrade MDS; Microbial Pathogenicity Laboratory, CEUMA University, São Luís, MA, Brazil., de Santana AVS; Microbial Pathogenicity Laboratory, CEUMA University, São Luís, MA, Brazil., da Silva LCT; Microbial Pathogenicity Laboratory, CEUMA University, São Luís, MA, Brazil., Sá GC; Microbial Pathogenicity Laboratory, CEUMA University, São Luís, MA, Brazil., Zafred IF; Microbial Pathogenicity Laboratory, CEUMA University, São Luís, MA, Brazil., Moreira PHA; Dental Laboratory, CEUMA University, São Luís, MA, Brazil., Monteiro CA; Laboratory of Research and Study in Microbiology, Federal Institute of Education, Science and Technology of the Maranhão (IFMA), São Luís, MA, Brazil., da Silva LCN; Microbial Pathogenicity Laboratory, CEUMA University, São Luís, MA, Brazil., Abreu AG; Microbial Pathogenicity Laboratory, CEUMA University, São Luís, MA, Brazil. Electronic address: afonso.abreu@ceuma.br.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2024 Apr; Vol. 173, pp. 116389. Date of Electronic Publication: 2024 Mar 11.
DOI: 10.1016/j.biopha.2024.116389
Abstrakt: Staphylococcus aureus is one of the most common bacterial isolates found in wounds. Thus, innovative dressings, such as hydrogels, are interesting vehicles for incorporating bioactive compounds like those from Melaleuca alternifolia essential oil (MaEO). In this study, we evaluated the antimicrobial and anti-inflammatory potential of MaEO incorporated into an alginate and chitosan hydrogel for treating wounds infected by S. aureus. The hydrogel incorporated with MaEO 1% (HMa 1%) was homogeneous with a bright pale-yellow color and the characteristic smell of Melaleuca. The incorporation of MaEO 1% does not affect the stability of the hydrogel, which was stable up to 90 days of storage. The Scanning electron microscopy analysis revealed that hydrogels showed irregular surfaces and interconnected porous structures with accumulations of oil crystals distributed throughout the formulation. HMa 1% has a high moisture content (95.1%) and can absorb simulated wound fluid. Regarding the antimicrobial effects, HMa 1% reduced the growth of S. aureus ATCC 6538 in both in vitro conditions and in an ex vivo model of wounds using porcine skin. In addition, the dairy topical treatment of murine skin lesions with HMa 1% induced a significant reduction of the wound area, inflammation score, and bacterial load, as well as tissue re-epithelialization and modulation of inflammatory mediators. Therefore, hydrogel incorporated with MaEO 1% has excellent potential to be used in the pharmacotherapy of infected wounds.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: