A flavonoid metabolon: cytochrome b 5 enhances B-ring trihydroxylated flavan-3-ols synthesis in tea plants.

Autor: Ruan H; School of Life Science, Anhui Agricultural University, Hefei, Anhui, 230036, China.; State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, Anhui, 230036, China., Gao L; School of Life Science, Anhui Agricultural University, Hefei, Anhui, 230036, China., Fang Z; School of Life Science, Anhui Agricultural University, Hefei, Anhui, 230036, China., Lei T; School of Life Science, Anhui Agricultural University, Hefei, Anhui, 230036, China., Xing D; School of Biological and Environmental Engineering, Chaohu University, Chaohu, Anhui, 238024, China., Ding Y; School of Life Science, Anhui Agricultural University, Hefei, Anhui, 230036, China., Rashid A; School of Life Science, Anhui Agricultural University, Hefei, Anhui, 230036, China.; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, 212013, China., Zhuang J; College of Tea Science, Guizhou University, Guiyang, Guizhou, 550025, China., Zhang Q; School of Life Science, Anhui Agricultural University, Hefei, Anhui, 230036, China., Gu C; School of Life Science, Anhui Agricultural University, Hefei, Anhui, 230036, China., Qian W; School of Life Science, Anhui Agricultural University, Hefei, Anhui, 230036, China., Zhang N; School of Life Science, Anhui Agricultural University, Hefei, Anhui, 230036, China., Qian T; School of Life Science, Anhui Agricultural University, Hefei, Anhui, 230036, China., Li K; College of Humanities and Social Development, Nanjing Agriculture University, Nanjing, Jiangsu, 210095, China., Xia T; State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, Anhui, 230036, China., Wang Y; School of Life Science, Anhui Agricultural University, Hefei, Anhui, 230036, China.; State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, Anhui, 230036, China.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2024 Jun; Vol. 118 (6), pp. 1793-1814. Date of Electronic Publication: 2024 Mar 10.
DOI: 10.1111/tpj.16710
Abstrakt: Flavan-3-ols are prominent phenolic compounds found abundantly in the young leaves of tea plants. The enzymes involved in flavan-3-ol biosynthesis in tea plants have been extensively investigated. However, the localization and associations of these numerous functional enzymes within cells have been largely neglected. In this study, we aimed to investigate the synthesis of flavan-3-ols in tea plants, particularly focusing on epigallocatechin gallate. Our analysis involving the DESI-MSI method to reveal a distinct distribution pattern of B-ring trihydroxylated flavonoids, primarily concentrated in the outer layer of buds. Subcellular localization showed that CsC4H, CsF3'H, and CsF3'5'H localizes endoplasmic reticulum. Protein-protein interaction studies demonstrated direct associations between CsC4H, CsF3'H, and cytoplasmic enzymes (CHS, CHI, F3H, DFR, FLS, and ANR), highlighting their interactions within the biosynthetic pathway. Notably, CsF3'5'H, the enzyme for B-ring trihydroxylation, did not directly interact with other enzymes. We identified cytochrome b 5 isoform C serving as an essential redox partner, ensuring the proper functioning of CsF3'5'H. Our findings suggest the existence of distinct modules governing the synthesis of different B-ring hydroxylation compounds. This study provides valuable insights into the mechanisms underlying flavonoid diversity and efficient synthesis and enhances our understanding of the substantial accumulation of B-ring trihydroxylated flavan-3-ols in tea plants.
(© 2024 Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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