Establishment of a hydrodynamic delivery system in ducks.

Autor: Zhao Z; Department of Pathology and Institute of Molecular Pathology, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China.; School of Pharmacy, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China., Zhu J; School of Pharmacy, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China., Zhou L; School of Pharmacy, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China., Sun N; School of Pharmacy, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China., Chang K; School of Pharmacy, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China., Hu X; School of Pharmacy, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China.; Institute of Translational Medicine, Jiangxi Medical College,, Nanchang University, Nanchang, 330031, People's Republic of China., Hu Y; School of Pharmacy, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China.; Institute of Translational Medicine, Jiangxi Medical College,, Nanchang University, Nanchang, 330031, People's Republic of China., Ren M; School of Pharmacy, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China., Cheng Y; School of Pharmacy, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China., Xu D; Institute of Translational Medicine, Jiangxi Medical College,, Nanchang University, Nanchang, 330031, People's Republic of China., Xin H; Institute of Translational Medicine, Jiangxi Medical College,, Nanchang University, Nanchang, 330031, People's Republic of China., Zhang C; Department of Pathology and Institute of Molecular Pathology, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China. cbzhang@ncu.edu.cn.; School of Pharmacy, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China. cbzhang@ncu.edu.cn.; Basic Research and Innovation Center for the Targeted Therapeutics of Solid Tumors, Ministry of Education, Jiangxi Medical College, Nanchang University, Nanchang, 330031, People's Republic of China. cbzhang@ncu.edu.cn.
Jazyk: angličtina
Zdroj: Transgenic research [Transgenic Res] 2024 Apr; Vol. 33 (1-2), pp. 35-46. Date of Electronic Publication: 2024 Mar 09.
DOI: 10.1007/s11248-024-00377-x
Abstrakt: Chronic hepatitis B virus (HBV) poses a significant global health challenge as it can lead to acute or chronic liver disease and hepatocellular carcinoma (HCC). To establish a safety experimental model, a homolog of HBV-duck HBV (DHBV) is often used for HBV research. Hydrodynamic-based gene delivery (HGD) is an efficient method to introduce exogenous genes into the liver, making it suitable for basic research. In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous genes could be expressed in a short time. Further, the application of the duck HGD model on DHBV treatment was investigated by transferring representative anti-HBV genes IFNα and IFNγ into DHBV-infected ducks. Delivery of plasmids expressing IFNα and IFNγ inhibited DHBV infection and we established a novel efficient HGD method in ducks, which could be useful for drug screening of new genes, mRNAs and proteins for anti-HBV treatment.
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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