The possible protective effect of l-cysteine in a rat model of sciatic nerve ischemia-reperfusion: A possible role for NRF1 and Caspase 3; Biochemical, Histological, and Immunohistochemical study.

Autor: Hafez SMNA; Histology and Cell Biology Department, Minia University, Faculty of Medicine, Minia 61111, Egypt. Electronic address: sara.abdelhafez@mu.edu.eg., Abdelhafez EMN; Department of Medicinal Chemistry, Faculty of Pharmacy, Minia University, Faculty of Medicine, Minia 61111, Egypt. Electronic address: shimaanaguib_80@mu.edu.eg.
Jazyk: angličtina
Zdroj: Journal of chemical neuroanatomy [J Chem Neuroanat] 2024 Apr; Vol. 137, pp. 102412. Date of Electronic Publication: 2024 Mar 07.
DOI: 10.1016/j.jchemneu.2024.102412
Abstrakt: Organ damage brought on by ischemia is exacerbated by the reperfusion process. L-cysteine is a semi-essential amino acid that acts as a substrate for cystathionine-β-synthase in the central nervous system. The aim of this study was to investigate the possible protective effects of L- cysteine against the structural and biochemical changes that occur in the rat sciatic nerve after ischemia reperfusion (I/R) and to address some of the underlying mechanisms of these effects. Rats were divided into 4 groups: sham, l-cysteine, I/R, and l-cysteine- I/R groups. Specimens of sciatic nerve were processed for biochemical, histological, and immunohistochemical assessment. The results showed in I/R group, a significant increase in malondialdehyde with a significant decrease in both Nuclear respiratory factor-1 (NRF1) and superoxide dismutase levels. Moreover, with histological alteration. There was a significant increase in the mean surface area fraction of anti-caspase immunopositive cells as well as a significantdecrease in mean surface area fraction of anti-CD 34 immunopositive cells. In contrast, the l-cysteine- I/R group showed amelioration of these biochemical, structural, and immunohistochemical changes. To the best of our knowledge, this is the first study showed the protective effects of l-cysteine in sciatic nerve I/R via NRF1and caspase 3 modulation as well as telocyte activation.
Competing Interests: Declaration of Competing Interest There is no conflict of interests disclosed.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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