Phenotypic, molecular detection, and Antibiotic Resistance Profile (MDR and XDR) of Aeromonas hydrophila isolated from Farmed Tilapia zillii and Mugil cephalus.

Autor: Ayoub HF; Department of Fish Health and Management, Central Laboratory for Aquaculture Research (CLAR), Agricultural Research Center, Abo-Hammad, Sharqia, Abbassa, 44662, Egypt. DrHalaclar16@gmail.com., Khafagy AR; Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Ain Shams University, Cairo, Egypt., Esawy AM; Department of Microbiology, Animal Health Research Institute, Mansoura branch, Mansoura, Egypt., El-Moaty NA; Department of Microbiology, Animal Health Research Institute, Mansoura branch, Mansoura, Egypt., Alwutayd KM; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia., Mansour AT; Fish and Animal Production and Aquaculture Department, College of Agriculture and Food Sciences, King Faisal University, P.O. Box 420, Al-Ahsa, 31982, Saudi Arabia. amansour@kfu.edu.sa.; Fish and Animal Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria, 21531, Egypt. amansour@kfu.edu.sa., Ibrahim RA; Microbiology Department, National Institute of Oceanography and Fisheries (NIOF), Cairo, Egypt., Abdel-Moneam DA; Department of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt., El-Tarabili RM; Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.
Jazyk: angličtina
Zdroj: BMC veterinary research [BMC Vet Res] 2024 Mar 08; Vol. 20 (1), pp. 84. Date of Electronic Publication: 2024 Mar 08.
DOI: 10.1186/s12917-024-03942-y
Abstrakt: In the present study, Aeromonas hydrophila was isolated from Tilapia zillii and Mugil cephalus samples collected during different seasons from various Suez Canal areas in Egypt. The prevalence of A. hydrophila, virulence genes, and antibiotic resistance profile of the isolates to the commonly used antibiotics in aquaculture were investigated to identify multiple drug resistance (MDR) and extensive drug-resistant (XDR) strains. In addition, a pathogenicity test was conducted using A. hydrophila, which was isolated and selected based on the prevalence of virulence and resistance genes, and morbidity of natural infected fish. The results revealed that A. hydrophila was isolated from 38 of the 120 collected fish samples (31.6%) and confirmed phenotypically and biochemically. Several virulence genes were detected in retrieved A. hydrophila isolates, including aerolysin aerA (57.9%), ser (28.9%), alt (26.3%), ast (13.1%), act (7.9%), hlyA (7.9%), and nuc (18.4%). Detection of antibiotic-resistant genes revealed that all isolates were positive for bla pse1 (100%), bla SHV (42.1%), tetA (60.5%), and sul1 (42.1%). 63.1% of recovered isolates were considered MDR, while 28.9% of recovered isolates were considered XDR. Some isolates harbor both virulence and MDR genes; the highest percentage carried 11, followed by isolates harboring 9 virulence and resistance genes. It could be concluded that the high prevalence of A. hydrophila in aquaculture species and their diverse antibiotic resistance and virulence genes suggest the high risk of Aeromonas infection and could have important implications for aquaculture and public health.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje