Unveiling biological activities of biosynthesized starch/silver-selenium nanocomposite using Cladosporium cladosporioides CBS 174.62.

Autor: Elkady FM; Microbiology and Immunology Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, 11884, Egypt., Hashem AH; Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt., Salem SS; Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt. salemsalahsalem@azhar.edu.eg., El-Sayyad GS; Department of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian (ACU), Giza, Egypt. Gharieb.S.Elsayyad@eaea.org.eg.; Department of Microbiology and Immunology, Faculty of Pharmacy, Galala University, New Galala City, Suez, Egypt. Gharieb.S.Elsayyad@eaea.org.eg.; Drug Microbiology Lab., Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt. Gharieb.S.Elsayyad@eaea.org.eg., Tawab AA; Department of Microbiology and Immunology, Faculty of Medicine, Al-Azhar University, Cairo, 11884, Egypt., Alkherkhisy MM; Department of Microbiology and Immunology, Faculty of Medicine, Al-Azhar University, Cairo, 11884, Egypt., Abdulrahman MS; Microbiology and Immunology Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, 11884, Egypt.
Jazyk: angličtina
Zdroj: BMC microbiology [BMC Microbiol] 2024 Mar 08; Vol. 24 (1), pp. 78. Date of Electronic Publication: 2024 Mar 08.
DOI: 10.1186/s12866-024-03228-1
Abstrakt: Background and Objectives: Microbial cells capability to tolerate the effect of various antimicrobial classes represent a major worldwide health concern. The flexible and multi-components nanocomposites have enhanced physicochemical characters with several improved properties. Thus, different biological activities of biosynthesized starch/silver-selenium nanocomposite (St/Ag-Se NC) were assessed.
Methodology: The St/Ag-Se NC was biosynthesized using Cladosporium cladosporioides CBS 174.62 (C. cladosporioides) strain. The shape and average particle size were investigated using scanning electron microscope (SEM) and high-resolution transmission electron microscope (HR-TEM), respectively. On the other hand, the St/Ag-Se NC effect on two cancer cell lines and red blood cells (RBCs) was evaluated and its hydrogen peroxide (H 2 O 2 ) scavenging effect was assessed. Moreover, its effects on various microbial species in both planktonic and biofilm growth forms were examined.
Results: The St/Ag-Se NC was successfully biosynthesized with oval and spherical shape and a mean particle diameter of 67.87 nm as confirmed by the HR-TEM analysis. St/Ag-Se NC showed promising anticancer activity toward human colorectal carcinoma (HCT-116) and human breast cancer (MCF-7) cell lines where IC 50 were 21.37 and 19.98 µg/ml, respectively. Similarly, little effect on RBCs was observed with low nanocomposite concentration. As well, the highest nanocomposite H 2 O 2 scavenging activity (42.84%) was recorded at a concentration of 2 mg/ml. Additionally, Staphylococcus epidermidis (S. epidermidis) ATCC 12,228 and Candida albicans (C. albicans) ATCC 10,231 were the highly affected bacterial and fungal strains with minimum inhibitory concentrations (MICs) of 18.75 and 50 µg/ml, respectively. Moreover, the noticeable effect of St/Ag-Se NC on microbial biofilm was concentration dependent. A high biofilm suppression percentage, 87.5% and 68.05%, were recorded with S. epidermidis and Staphylococcus aureus (S. aureus) when exposed to 1 mg/ml and 0.5 mg/ml, respectively.
Conclusion: The biosynthesized St/Ag-Se NC showed excellent antioxidant activity, haemocompatibility, and anti-proliferative effect at low concentrations. Also, it exhibited promising antimicrobial and antibiofilm activities.
(© 2024. The Author(s).)
Databáze: MEDLINE
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