Molecular characterization and demographic insights into soybean bud borer (Lepidoptera: Tortricidae) in Brazil.

Autor:	Fernandes DS; Department of Entomology and Acarology, 'Luiz de Queiroz' College of Agriculture, University of São Paulo (ESALQ/USP), Piracicaba, São Paulo, Brazil., Horikoshi RJ; Department of Entomology and Acarology, 'Luiz de Queiroz' College of Agriculture, University of São Paulo (ESALQ/USP), Piracicaba, São Paulo, Brazil.; Department of Regulatory Science, Bayer Crop Science Brazil, São Paulo, São Paulo, Brazil., Dourado PM; Department of Regulatory Science, Bayer Crop Science Brazil, São Paulo, São Paulo, Brazil., Ovejero RFL; Department of Regulatory Science, Bayer Crop Science Brazil, São Paulo, São Paulo, Brazil., Berger GU; Department of Regulatory Science, Bayer Crop Science Brazil, São Paulo, São Paulo, Brazil., Savaris M; Department of Entomology and Acarology, 'Luiz de Queiroz' College of Agriculture, University of São Paulo (ESALQ/USP), Piracicaba, São Paulo, Brazil., Brown JW; Department of Entomology, National Museum of Natural History, Smithsonian Institution, Washington, DC, USA., Corrêa AS; Department of Entomology and Acarology, 'Luiz de Queiroz' College of Agriculture, University of São Paulo (ESALQ/USP), Piracicaba, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of insect science (Online) [J Insect Sci] 2024 Mar 01; Vol. 24 (2).
DOI:	10.1093/jisesa/ieae019
Abstrakt:	The soybean bud borer, a soybean pest in Brazil, was initially identified as Crocidosema aporema (Walsingham 1914) (Lepidoptera: Tortricidae). Outbreaks of this species have recently increased, but identification of this pest remains uncertain, and the historical factors associated with its geographic distribution in Brazil are little known. Here, we conducted a species characterization and phylogeographic analysis based on molecular and morphological evidence. Ninety individuals of bud-borers Lepidoptera were collected in different regions of Brazil. We sequenced COI and COII mitochondrial genes and examined wing patterns and male genital morphology. DNA barcoding approach revealed that 10 individuals were Argyrotaenia sphaleropa (Meyrick 1909) (Lepidoptera: Tortricidae) and 80 were a species of the genus Crocidosema Zeller. The morphology of the adult genitalia and wings proved to be insufficient to confirm the identification of Brazilian individuals as C. aporema, a species originally described from a high-elevation site in Costa Rica. Furthermore, the genetic distance between putative C. aporema specimens from Brazil and Costa Rica (ranging from 5.2% to 6.4%) supports the hypothesis that the Brazilian specimens are not referable to C. aporema. Our analysis revealed a single genetic strain (i.e., species) with low genetic diversity on soybean crops. We found no indication that the genetic structure was related to geographic distance among populations or edaphoclimatic regions. The population expansion of the soybean bud borer coincides with the increase in the area of soybean production in Brazil, suggesting that expanded soybean farming has allowed a significant increase in the effective population size of this pest. (© The Author(s) 2024. Published by Oxford University Press on behalf of Entomological Society of America.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu