Potential of using medicinal plant extracts as photosensitizers for antimicrobial photodynamic therapy.

Autor: Mikulich AV; State Scientific Institution 'B.I. Stepanov Institute of Physics of the National Academy of Sciences of Belarus', Minsk, Republic of Belarus., Plavskii VY; State Scientific Institution 'B.I. Stepanov Institute of Physics of the National Academy of Sciences of Belarus', Minsk, Republic of Belarus., Tretyakova AI; State Scientific Institution 'B.I. Stepanov Institute of Physics of the National Academy of Sciences of Belarus', Minsk, Republic of Belarus., Nahorny RK; State Scientific Institution 'B.I. Stepanov Institute of Physics of the National Academy of Sciences of Belarus', Minsk, Republic of Belarus., Sobchuk AN; State Scientific Institution 'B.I. Stepanov Institute of Physics of the National Academy of Sciences of Belarus', Minsk, Republic of Belarus., Dudchik NV; Republican Unitary Enterprise «Scientific Practical Centre of Hygiene», Minsk, Republic of Belarus., Emeliyanova OA; Republican Unitary Enterprise «Scientific Practical Centre of Hygiene», Minsk, Republic of Belarus., Zhabrouskaya AI; Republican Unitary Enterprise «Scientific Practical Centre of Hygiene», Minsk, Republic of Belarus., Plavskaya LG; State Scientific Institution 'B.I. Stepanov Institute of Physics of the National Academy of Sciences of Belarus', Minsk, Republic of Belarus., Ananich TS; State Scientific Institution 'B.I. Stepanov Institute of Physics of the National Academy of Sciences of Belarus', Minsk, Republic of Belarus., Dudinova ON; State Scientific Institution 'B.I. Stepanov Institute of Physics of the National Academy of Sciences of Belarus', Minsk, Republic of Belarus., Leusenka IA; State Scientific Institution 'B.I. Stepanov Institute of Physics of the National Academy of Sciences of Belarus', Minsk, Republic of Belarus., Yakimchuk SV; State Scientific Institution 'B.I. Stepanov Institute of Physics of the National Academy of Sciences of Belarus', Minsk, Republic of Belarus., Svechko AD; State Scientific Institution 'B.I. Stepanov Institute of Physics of the National Academy of Sciences of Belarus', Minsk, Republic of Belarus., Tien TQ; Institute of Materials Science, Vietnam Academy of Science and Technology, Hanoi, Vietnam., Tong QC; Institute of Materials Science, Vietnam Academy of Science and Technology, Hanoi, Vietnam., Nguyen TP; School of Engineering Physics, Hanoi University of Science and Technology, Hanoi, Vietnam.
Jazyk: angličtina
Zdroj: Photochemistry and photobiology [Photochem Photobiol] 2024 Nov-Dec; Vol. 100 (6), pp. 1833-1847. Date of Electronic Publication: 2024 Mar 08.
DOI: 10.1111/php.13935
Abstrakt: Antimicrobial photodynamic therapy (APDT) is a promising approach to overcome antimicrobial resistance. However, for widespread implementation of this approach, approved photosensitizers are needed. In this study, we used commercially available preparations (Calendulae officinalis floridis extract, Chamomillae recutitae floridis extract, Achillea millefolii herbae extract; Hypericum perforatum extract; Eucalyptus viminalis folia extract) as photosensitizers for inactivation of gram-negative (Pseudomonas aeruginosa) and gram-positive (Staphylococcus aureus) bacteria. Spectral-luminescent analysis has shown that the major chromophores are of chlorophyll (mainly chlorophyll a and b) and hypericin nature. The extracts are efficient generators of singlet oxygen with quantum yield (γ Δ ) from 0.40 to 0.64 (reference compound, methylene blue with γ Δ = 0.52). In APDT assays, bacteria before irradiation were incubated with extracts for 30 min. After irradiation and 24 h of incubation, colony-forming units (CFU) were counted. Upon exposure of P. aeruginosa to radiation of 405 nm, 590 nm, and 660 nm at equal energy dose of 30 J/cm 2 (irradiance - 100 mW/cm 2 , exposure time - 5 min), the most pronounced effect is observed with blue light (>3 log 10 reduction); in case of S. aureus, the effect is approximately equivalent for light of indicated wavelengths and dose (>4 log 10 reduction).
(© 2024 American Society for Photobiology.)
Databáze: MEDLINE
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