Reversible oxidative dimerization of 4-thiouridines in tRNA isolates.
| Autor: | Bessler L; Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University Mainz Staudingerweg 5 55128 Mainz Germany mhelm@uni-mainz.de., Groß J; Department of Chemistry, Johannes Gutenberg University Mainz Duesbergweg 10-14 55128 Mainz Germany., Kampf CJ; Department of Chemistry, Johannes Gutenberg University Mainz Duesbergweg 10-14 55128 Mainz Germany., Opatz T; Department of Chemistry, Johannes Gutenberg University Mainz Duesbergweg 10-14 55128 Mainz Germany., Helm M; Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University Mainz Staudingerweg 5 55128 Mainz Germany mhelm@uni-mainz.de. |
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| Jazyk: | angličtina |
| Zdroj: | RSC chemical biology [RSC Chem Biol] 2024 Feb 01; Vol. 5 (3), pp. 216-224. Date of Electronic Publication: 2024 Feb 01 (Print Publication: 2024). |
| DOI: | 10.1039/d3cb00221g |
| Abstrakt: | The occurrence of non-canonical nucleoside structures in RNA of biological or synthetic origin has encountered several recent boosts in attention, namely in the context of RNA modifications, and with an eye to RNA vaccines. New nucleoside structures introduce added functionality and function into biopolymers that are otherwise rather homogenous in their chemical structure. Here, we report the discovery of a presumed RNA modification that was identified by combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) with stable isotope labelling as a dimer of the known RNA modification 4-thiouridine (s 4 U). The disulfide-linked structure, which had previously been synthetically introduced into RNA, was here formed spontaneously in isolates of E. coli tRNA. Judicious application of stable isotope labelling suggested that this presumed new RNA modification was rather generated ex vivo by oxidation with ambient oxygen. These findings do not only underscore the need for caution in the discovery of new RNA modifications with respect to artifacts, but also raise awareness of an RNA vulnerability, especially to oxidative damage, during its transport or storage. Competing Interests: Mark Helm is a consultant for Moderna Inc. (This journal is © The Royal Society of Chemistry.) |
| Databáze: | MEDLINE |
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