Caffeic acid protects against l-methionine induced reduction in neurogenesis and cognitive impairment in a rat model.

Autor:	Sirichoat A; Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.; Neurogenesis Research Group, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand., Dornlakorn O; Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.; Neurogenesis Research Group, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand., Saenno R; Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.; Neurogenesis Research Group, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand., Aranarochana A; Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.; Neurogenesis Research Group, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand., Sritawan N; Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.; Neurogenesis Research Group, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand., Pannangrong W; Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand., Wigmore P; School of Life Sciences, Medical School, Queen's Medical Centre, The University of Nottingham, Nottingham, United Kingdom., Welbat JU; Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.; Neurogenesis Research Group, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 Feb 25; Vol. 10 (5), pp. e26919. Date of Electronic Publication: 2024 Feb 25 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e26919
Abstrakt:	l-methionine (L-met) is a substantial non-polar amino acid for normal development. L-met is converted to homocysteine that leads to hyperhomocysteinemia and subsequent excessive homocysteine in serum resulting in stimulating oxidative stress and vascular dementia. Several studies have found that hyperhomocysteine causes neuronal cell damage, which leads to memory impairment. Caffeic acid is a substrate in phenolic compound discovered in plant biosynthesis. Caffeic acid contains biological antioxidant and neuroprotective properties. The neuroprotective reaction of caffeic acid can protect against the brain disruption from hydrogen peroxide produced by oxidative stress. It also enhances GSH and superoxide dismutase activities, which protect against neuron cell loss caused by oxidative stress in the hippocampus. Hence, we investigated the protective role of caffeic acid in hippocampal neurogenesis and cognitive impairment induced by L-met in rats. Six groups of Sprague Dawley rats were assigned including control, L-met (1.7 g/kg/day), caffeic acid (20, 40 mg/kg), and L-met + caffeic acid (20, 40 mg/kg) groups. Spatial and recognition memories were subsequently examined using novel object location (NOL) and novel object recognition (NOR) tests. Moreover, the immunofluorescence technique was performed to detect Ki-67/RECA-1, bromodeoxyuridine (BrdU)/NeuN and p21 markers to represent hippocampal neurogenesis changes. The results revealed decreases in vasculature related cell proliferation and neuronal cell survival. By contrast, cell cycle arrest was increased in the L-met group. These results showed the association of the spatial and recognition memory impairments. However, the deterioration can be restored by co-administration with caffeic acid. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu