Chromatin Immunoprecipitation (ChIP) Using Drosophila Antennal and Brain Samples.
| Autor: | Du C; Department of Biology, Duke University, Durham, North Carolina 27708, USA., Volkan P; Department of Biology, Duke University, Durham, North Carolina 27708, USA pc72@duke.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2025 Jan 02; Vol. 2025 (1), pp. pdb.prot108142. Date of Electronic Publication: 2025 Jan 02. |
| DOI: | 10.1101/pdb.prot108142 |
| Abstrakt: | Chromatin immunoprecipitation (ChIP) is a common approach for studying the binding pattern of proteins on DNA sequences or the landscape of histones with different marks throughout the genome. ChIP is used on various organisms, including Drosophila This protocol provides a detailed overview of the immunoprecipitation portion of a ChIP procedure from samples of Drosophila nervous systems, specifically antennae and brains, that have already been fixed and sheared. These methods can be applied to other tissues of interest after optimizing for sample size and other relevant parameters. (© 2025 Cold Spring Harbor Laboratory Press.) |
| Databáze: | MEDLINE |
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