| Autor: |
Ochs F; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK., Green C; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK., Szczurek AT; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK., Pytowski L; Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK., Kolesnikova S; Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), 1030 Vienna Austria.; Vienna BioCenter PhD Program, Doctoral School of the University of Vienna and Medical University of Vienna, 1030 Vienna Austria., Brown J; MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, UK., Gerlich DW; Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), 1030 Vienna Austria., Buckle V; MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, UK., Schermelleh L; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK., Nasmyth KA; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK. |
| Abstrakt: |
Eukaryotic genomes are organized by loop extrusion and sister chromatid cohesion, both mediated by the multimeric cohesin protein complex. Understanding how cohesin holds sister DNAs together, and how loss of cohesion causes age-related infertility in females, requires knowledge as to cohesin's stoichiometry in vivo. Using quantitative super-resolution imaging, we identified two discrete populations of chromatin-bound cohesin in postreplicative human cells. Whereas most complexes appear dimeric, cohesin that localized to sites of sister chromatid cohesion and associated with sororin was exclusively monomeric. The monomeric stoichiometry of sororin:cohesin complexes demonstrates that sister chromatid cohesion is conferred by individual cohesin rings, a key prediction of the proposal that cohesion arises from the co-entrapment of sister DNAs. |
