| Autor: |
Klemm EJ; Wellcome Sanger Institute, Cambridge, UK., Nisar MI; Department of Paediatrics and Child Health, Aga Khan University, Karachi, Pakistan., Bawn M; Quadram Institute Bioscience, Norwich, UK.; Faculty of Biological Sciences, University of Leeds, Leeds, UK., Nasrin D; Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USA., Qamar FN; Department of Paediatrics and Child Health, Aga Khan University, Karachi, Pakistan., Page A; Quadram Institute Bioscience, Norwich, UK., Qadri F; Department of Paediatrics and Child Health, Aga Khan University, Karachi, Pakistan., Shakoor S; Department of Paediatrics and Child Health, Aga Khan University, Karachi, Pakistan., Zaidi AK; Department of Paediatrics and Child Health, Aga Khan University, Karachi, Pakistan.; Bill & Melinda Gates Foundation, Seattle, Washington, USA., Levine MM; Faculty of Biological Sciences, University of Leeds, Leeds, UK., Dougan G; Cambridge Institute of Therapeutic Immunology & Infectious Disease, University of Cambridge, England, UK., Kingsley RA; Quadram Institute Bioscience, Norwich, UK.; School of Biological Sciences, University of East Anglia, Norwich, UK. |
| Abstrakt: |
Aeromonas spp. are associated with a number of infectious syndromes in humans including gastroenteritis and dysentery. Our understanding of the genetic diversity, population structure, virulence determinants and antimicrobial resistance of the genus has been limited by a lack of sequenced genomes linked to metadata. We performed a comprehensive analysis of the whole genome sequences of 447 Aeromonas isolates from children in Karachi, Pakistan, with moderate-to-severe diarrhoea (MSD) and from matched controls without diarrhoea that were collected as part of the Global Enteric Multicenter Study (GEMS). Human-associated Aeromonas isolates exhibited high species diversity and extensive antimicrobial and virulence gene content. Aeromonas caviae , A. dhankensis , A. veronii and A. enteropelogenes were all significantly associated with MSD in at least one cohort group. The maf2 and lafT genes that encode components of polar and lateral flagella, respectively, exhibited a weak association with isolates originating from cases of gastroenteritis. |
