Antibodies from chlamydia-infected individuals facilitate phagocytosis via Fc receptors.

Autor:	Hybiske K; Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA., Paktinat S; Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA., Newman K; Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA., Patton D; Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA., Khosropour C; Department of Epidemiology, University of Washington, Seattle, Washington, USA., Roxby AC; Department of Medicine, University of Washington, Seattle, Washington, USA.; Department of Global Health, University of Washington, Seattle, Washington, USA., Mugo NR; Department of Global Health, University of Washington, Seattle, Washington, USA.; Centre for Clinical Research, Kenya Medical Research Institute, Nairobi, Kenya., Oluoch L; Centre for Clinical Research, Kenya Medical Research Institute, Nairobi, Kenya., Ngure K; Department of Global Health, University of Washington, Seattle, Washington, USA.; School of Public Health, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya., Suchland R; Department of Medicine, University of Washington, Seattle, Washington, USA., Hladik F; Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA., Vojtech L; Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA.
Jazyk:	angličtina
Zdroj:	Infection and immunity [Infect Immun] 2024 Apr 09; Vol. 92 (4), pp. e0050323. Date of Electronic Publication: 2024 Mar 07.
DOI:	10.1128/iai.00503-23
Abstrakt:	Non-neutralizing functions of antibodies, including phagocytosis, may play a role in Chlamydia trachomatis (CT) infection, but these functions have not been studied and assays are lacking. We utilized a flow-cytometry-based assay to determine whether serum samples from a well-characterized cohort of CT-infected and naïve control individuals enhanced phagocytosis via Fc-receptor-expressing THP-1 cells, and whether this activity correlated with antibody titers. Fc-receptor-mediated phagocytosis was detected only in CT+ donors. Phagocytosis generally did not correlate well with antibody titer. In addition, we found that complement from both CT+ and negative individuals enhanced phagocytosis of CT into primary neutrophils. These results suggest that anti-CT antibodies can have functions that are not reflected by titer. This method could be used to quantitively measure Fc-receptor-mediated function of anti-CT antibodies or complement activity and could reveal new immune correlates of protection. Competing Interests: N.R.M. and K.N. report receiving research funding under the Merck Investigator Sponsored Program. The other authors report no conflict of interest.
Databáze:	MEDLINE
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