| Autor: |
Buneeva OA; Institute of Biomedical Chemistry, Moscow, Russia., Kapitsa IG; Institute of Biomedical Chemistry, Moscow, Russia; Zakusov Institute of Pharmacology, Moscow, Russia., Kazieva LS; Institute of Biomedical Chemistry, Moscow, Russia., Vavilov NE; Institute of Biomedical Chemistry, Moscow, Russia., Zgoda VG; Institute of Biomedical Chemistry, Moscow, Russia., Medvedev AE; Institute of Biomedical Chemistry, Moscow, Russia. |
| Jazyk: |
angličtina |
| Zdroj: |
Biomeditsinskaia khimiia [Biomed Khim] 2024 Feb; Vol. 70 (1), pp. 25-32. |
| DOI: |
10.18097/PBMC20247001025 |
| Abstrakt: |
Isatin (indoldione-2,3) is an endogenous biological regulator found in the brain, peripheral tissues, and biological fluids of humans and animals. Its biological activity is realized via isatin-binding proteins, many of which were identified during proteomic profiling of the brain of mice and rats. A number of these proteins are related to the development of neurodegenerative diseases. Previously, using a model of experimental Parkinsonism induced by a seven-day course of rotenone injections, we have observed behavioral disturbances, as well as changes in the profile and relative content of brain isatin-binding proteins. In this study, we have investigated behavioral responses and the relative content of brain isatin-binding proteins in rats with rotenone-induced Parkinsonism 5 days after the last administration of this neurotoxin. Despite the elimination of rotenone, animals exhibited motor and coordination impairments. Proteomic profiling of isatin-binding proteins revealed changes in the relative content of 120 proteins (the relative content of 83 proteins increased and that of 37 proteins decreased). Comparison of isatin-binding proteins characterized by the changes in the relative content observed in the brain right after the last injection of rotenone (n=16) and 5 days later (n=11) revealed only two common proteins (glyceraldehyde-3-phosphate dehydrogenase and subunit B of V-type proton ATPase). However, most of these proteins are associated with neurodegeneration, including Parkinson's and Alzheimer's diseases. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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