Genomic characterization of a WHO critical priority isolate Enterobacter kobei ST2070 harboring OXA-10, KPC-2, and CTX-M-12 recovered from a water irrigation channel in Ecuador.
| Autor: | Corrales-Martínez J; UTA-RAM-One Health, Department of Food and Biotechnology Science and Engineering, Universidad Técnica de Ambato, Ambato, Ecuador., Jaramillo K; Centro de Referencia Nacional de Resistencia a los Antimicrobianos RAM, Instituto Nacional de Investigación en Salud Pública 'Dr. Leopoldo Izquieta Pérez' INSPI, Quito, Ecuador.; Facultad de Ciencias de la Salud, Universidad Técnica de Ambato, Ambato, Ecuador., Tadesse DA; U.S. Food &Drug Administration, Center for Veterinary Medicine, Office of Applied Science Laurel, MD 20708, USA., Satán C; Centro de Referencia Nacional de Resistencia a los Antimicrobianos RAM, Instituto Nacional de Investigación en Salud Pública 'Dr. Leopoldo Izquieta Pérez' INSPI, Quito, Ecuador., Villavicencio FX; Centro de Referencia Nacional de Resistencia a los Antimicrobianos RAM, Instituto Nacional de Investigación en Salud Pública 'Dr. Leopoldo Izquieta Pérez' INSPI, Quito, Ecuador.; Veterinary Medicine, Eugenio Espejo Faculty of Health Sciences, Universidad UTE, Quito, Ecuador., Sánchez-Gavilanes L; UTA-RAM-One Health, Department of Food and Biotechnology Science and Engineering, Universidad Técnica de Ambato, Ambato, Ecuador., Rivadeneira-Cueva B; UTA-RAM-One Health, Department of Food and Biotechnology Science and Engineering, Universidad Técnica de Ambato, Ambato, Ecuador., Balcázar JL; Catalan Institute for Water Research (ICRA), 17003 Girona, Spain.; University of Girona, 17004 Girona, Spain., Calero-Cáceres W; UTA-RAM-One Health, Department of Food and Biotechnology Science and Engineering, Universidad Técnica de Ambato, Ambato, Ecuador. |
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| Jazyk: | angličtina |
| Zdroj: | Heliyon [Heliyon] 2024 Feb 16; Vol. 10 (5), pp. e26379. Date of Electronic Publication: 2024 Feb 16 (Print Publication: 2024). |
| DOI: | 10.1016/j.heliyon.2024.e26379 |
| Abstrakt: | The discharge of untreated or partially treated wastewater can have detrimental impacts on the quality of water bodies, posing a significant threat to public health and the environment. In Ecuador, previous research indicates a high prevalence of antimicrobial resistant (AMR) bacteria in surface waters affected by human activities, including irrigation channels. In this study, we analyzed sediment samples collected from an irrigation channel utilized for agricultural purposes in northern Ecuador, using microbiological techniques and whole-genome sequencing (WGS). Our investigation revealed the first documented occurrence of E. kobei in Ecuador and the initial report of environmental E. kobei ST2070. Furthermore, we identified the coexistence of OXA-10-type class D β-lactamase and KPC-2-type class A β-lactamase in the E. kobei isolate (UTA41), representing the first report of such a phenomenon in this species. Additionally, we detected various antibiotic resistance genes in the E. kobei UTA41 isolate, including bla Competing Interests: William Calero-Caceres reports financial support and equipment, drugs, or supplies were provided by 10.13039/100016022Technical University of Ambato. Daniel A. Tadesse reports financial support and equipment, reagents, or supplies were provided by US 10.13039/100009210Food and Drug Administration. Katherine Jaramillo, Carolina Satan, Fernando X. Villavicencio reports financial support and equipment, drugs, or supplies were provided by National Institute for Research in Public Health (INSPI). Jose Luis Balcazar reports financial support was provided by Catalan Institute of Investigation of Water. (© 2024 The Authors.) |
| Databáze: | MEDLINE |
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