3D printed arrowroot starch-gellan scaffolds for wound healing applications.

Autor: Joseph A; Department of Bioscience & Engineering, National Institute of Technology Calicut, Kozhikode, Kerala 673601, India., Muhammad L F; Department of Bioscience & Engineering, National Institute of Technology Calicut, Kozhikode, Kerala 673601, India., S Vijayan A; School of Material Science and Engineering, National Institute of Technology Calicut, Kozhikode, Kerala 673601, India., Xavier J; Toxicology division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojapura, Trivandrum, Kerala, India., K B M; Toxicology division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojapura, Trivandrum, Kerala, India., Karthikeyan A; Department of Bioscience & Engineering, National Institute of Technology Calicut, Kozhikode, Kerala 673601, India., Gopinath N; Department of Bioscience & Engineering, National Institute of Technology Calicut, Kozhikode, Kerala 673601, India., P V M; Toxicology division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojapura, Trivandrum, Kerala, India., Nair BG; Department of Bioscience & Engineering, National Institute of Technology Calicut, Kozhikode, Kerala 673601, India. Electronic address: bgnair@nitc.ac.in.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Apr; Vol. 264 (Pt 1), pp. 130604. Date of Electronic Publication: 2024 Mar 05.
DOI: 10.1016/j.ijbiomac.2024.130604
Abstrakt: Skin, the largest organ in the body, blocks the entry of environmental pollutants into the system. Any injury to this organ allows infections and other harmful substances into the body. 3D bioprinting, a state-of-the-art technique, is suitable for fabricating cell culture scaffolds to heal chronic wounds rapidly. This study uses starch extracted from Maranta arundinacea (Arrowroot plant) (AS) and gellan gum (GG) to develop a bioink for 3D printing a scaffold capable of hosting animal cells. Field emission scanning electron microscopy (FE-SEM) and X-ray diffraction analysis (XRD) prove that the isolated AS is analogous to commercial starch. The cell culture scaffolds developed are superior to the existing monolayer culture. Infrared microscopy shows the AS-GG interaction and elucidates the mechanism of hydrogel formation. The physicochemical properties of the 3D-printed scaffold are analyzed to check the cell adhesion and growth; SEM images have confirmed that the AS-GG printed scaffold can support cell growth and proliferation, and the MTT assay shows good cell viability. Cell behavioral and migration studies reveal that cells are healthy. Since the scaffold is biocompatible, it can be 3D printed to any shape and structure and will biodegrade in the requisite time.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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